Autism spectrum disorder:difficulties in diagnosis and microRNA biomarkers  

作　　者：Bridget Martinez Philip V.Peplow 

机构地区：[1]Department of Pharmacology,University of Nevada-Reno,Reno,NV,USA [2]Department of Medicine,University of Nevada-Reno,Reno,NV,USA [3]Department of Anatomy,University of Otago,Dunedin,New Zealand

出　　处：《Neural Regeneration Research》2025年第10期2776-2786,共11页中国神经再生研究（英文版）

摘　　要：We performed a PubMed search for microRNAs in autism spectrum disorder that could serve as diagnostic biomarkers in patients and selected 17 articles published from January 2008 to December 2023,of which 4 studies were performed with whole blood,4 with blood plasma,5 with blood serum,1 with serum neural cell adhesion molecule L1-captured extracellular vesicles,1 with blood cells,and 2 with peripheral blood mononuclear cells.Most of the studies involved children and the study cohorts were largely males.Many of the studies had performed microRNA sequencing or quantitative polymerase chain reaction assays to measure microRNA expression.Only five studies had used real-time polymerase chain reaction assay to validate microRNA expression in autism spectrum disorder subjects compared to controls.The microRNAs that were validated in these studies may be considered as potential candidate biomarkers for autism spectrum disorder and include miR-500a-5p,-197-5p,-424-5p,-664a-3p,-365a-3p,-619-5p,-664a-3p,-3135a,-328-3p,and-500a-5p in blood plasma and miR-151a-3p,-181b-5p,-320a,-328,-433,-489,-572,-663a,-101-3p,-106b-5p,-19b-3p,-195-5p,and-130a-3p in blood serum of children,and miR-15b-5p and-6126 in whole blood of adults.Several important limitations were identified in the studies reviewed,and need to be taken into account in future studies.Further studies are warranted with children and adults having different levels of autism spectrum disorder severity and consideration should be given to using animal models of autism spectrum disorder to investigate the effects of suppressing or overexpressing specific microRNAs as a novel therapy.

关 键 词：autism spectrum disorder BIOMARKER blood cells blood plasma blood serum DIAGNOSIS MICRORNA peripheral blood mononuclear cells serum neural cell adhesion molecule L1-captured extracellular vesicles whole blood 

分 类 号：R749.94[医药卫生—神经病学与精神病学]