Thioredoxin pathway regulated live-cell synthesis of CdSe quantum dots in Saccharomyces cerevisiae  

作　　者：Xu Li Cai-Qin Wu Jia-Wei Tu Juan Kong Ling-Ling Yang Zhi-Xiong Xie Dai-Wen Pang 

机构地区：[1]College of Chemistry and Molecular Sciences,The Institute for Advanced Studies,College of Life Sciences,Wuhan University,Wuhan 430072,China [2]Research Center for Analytical Sciences,College of Chemistry,State Key Laboratory of Medicinal Chemical Biology,Tianjin Key Laboratory of Biosensing and Molecular Recognition,Frontiers Science Center for New Organic Matter,Frontiers Science Center for Cell Responses,and Haihe Laboratory of Sustainable Chemical Transformations,Nankai University,Tianjin 300071,China

出　　处：《Science China Chemistry》2024年第11期3851-3860,共10页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (22293030, 22293032);the National Key Research and Development Program of China (2019YFA0210100);the Fundamental Research Funds for the Central Universities of China (63211023)。

摘　　要：Currently, the application of synthetic biology to artificially manipulate and utilize organisms for the synthesis of desired products such as nanomaterials with excellent fluorescence properties is attracting considerable attention. However, it is still difficult to obtain designed products efficiently due to insufficient knowledge of the biosynthetic mechanisms. The thioredoxin(TRX) and glutathione(GSH) pathways are generally conserved thiol-reductase systems that protect organisms from oxidative stress and are involved in selenium(Se) metabolism. In this study, we revealed the pivotal role of cytoplasmic TRX pathway in regulating the metabolism of Na_(2)SeO_(3) during the live-cell synthesis of cadmium-selenium quantum dots(Cd Se QDs) in Saccharomyces cerevisiae by regulating the expression level of genes related to TRX pathway and measuring the intracellular content of selenocysteine(Se Cys). The determination of Se Cys metabolism in yeast with GSH pathway-related genes deleted demonstrated that the TRX pathway played a more significant role in Se Cys metabolism than GSH pathway. A 6.4-fold enhancement in the synthetic yield of Cd Se QDs was achieved through the overexpression of TRX pathway-related genes,improvement of synthetic procedure, and supplementation of GSH based on the understanding of biological metabolism.Exploring the mechanism of CdSe QDs live-cell synthesis facilitates the precise manipulation of biological processes for the synthesis of inorganic nanomaterials.

关 键 词：THIOREDOXIN GLUTATHIONE quantum dot live-cell synthesis selenium metabolism Saccharomyces cerevisiae 

分 类 号：Q78[生物学—分子生物学] TB383.1[一般工业技术—材料科学与工程]