抗仔猪腹泻大肠杆菌细菌素的筛选及全基因组测序分析  

Screening and whole-genome sequencing analysis of Escherichia coli bacteriocins against piglet diarrhea

作　　者：陆童 王云飞 蒋吕鹏 董文龙 李国江[1,3,4] 尹柏双 LU Tong;WANG Yunfei;JIANG Lvpeng;DONG Wenlong;LI Guojiang;YIN Baishuang(College of Veterinary Medicine,Jilin Institute of Agricultural Science and Technology,Jilin,Jilin Province 132109,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin Province 130118,China;Jilin Key Laboratory of Preventive Veterinary Medicine,Jilin,Jilin Province 132109,China;Jilin Province Pig Ecological Breeding and Disease Prevention and Control Science and Technology Innovation Center,Jilin,Jilin Province 132109,China;Jilin Province Collaborate Across Domains Technology Innovation Center of Pig Main Disease Prevention and Control,Jilin,Jilin Province 132109,China)

机构地区：[1]吉林农业科技学院动物医学院,吉林吉林132109 [2]吉林农业大学动物科学技术学院,吉林长春130118 [3]吉林省预防兽医学重点实验室,吉林吉林132109 [4]吉林省猪生态养殖及疫病防控科技创新中心,吉林吉林132109 [5]吉林省猪重要疫病防控跨区域合作科技创新中心,吉林吉林132109

出　　处：《中国饲料》2024年第21期26-31,共6页China Feed

基　　金：吉林省科技发展计划重点研发项目(20210202047NC)。

摘　　要：为筛选对大肠杆菌高效、稳定的乳酸菌细菌素,本试验从内蒙古自治区泡菜中分离鉴定一株植物乳杆菌,命名为LT-1,其所产细菌素pH为3时抑菌效果最好,且热稳定性较好,经100℃处理后,其抑菌活性略微减小,其所产细菌素对木瓜蛋白酶、胃蛋白酶和蛋白酶K较为敏感。LT-1所产细菌素对大肠杆菌ATCC25922及大肠杆菌临床分离株有良好的抑菌活性。对LT-1进行全基因组测序对其细菌素编码基因簇进行预测,LT-1所产细菌素的基因簇主要编码乳球菌素、肠球菌素和细菌素免疫蛋白。本研究所筛选的细菌素具有良好的抗菌活性,为大肠杆菌替抗产品的研发奠定良好的基础。Screening for efficient and stable lactic acid bacteria bacteriocins against Escherichia coli.A strain of Lactobacillus plantarum isolated and identified from kimchi in Inner Mongolia Autonomous Region,named LT-1,the bacteriocin produced had the best bacteriostatic effect when the pH was 3,and the thermal stability was good,after 100 ℃ treatment,its bacteriostatic activity was slightly reduced,and the bacteriocin produced was more sensitive to papain,pepsin and proteinase K.The bacteriocin produced by LT-1 had good bacteriostatic activity against Escherichia coli ATCC25922 and clinical isolates of Escherichia coli.Whole genome sequencing of LT-1 predicted the bacteriocin-coding gene cluster,which mainly encodes lactococcin,enterococcin,and bacteriocin immunoproteins.The bacteriocins screened in this institute had good antibacterial activity,which laid a good foundation for the research and development of Escherichia coli antiantibody products.

关 键 词：植物乳杆菌 大肠杆菌 仔猪腹泻 细菌素 全基因组测序 

分 类 号：S816.3[农业科学—饲料科学]