基于活细胞仪优化毕赤酵母发酵生产植酸酶研究  

Optimization of fermentation process for producing phytase by Pichia pastoris with an online biomass sensor

作　　者：徐鸿飞 李连伟 王凤超 李超 李玉强 王永红[1] XU Hongfei;LI Lianwei;WANG Fengchao;LI Chao;LI Yuqiang;WANG Yonghong(School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China;Qingdao Vland Biotech Inc.,Qingdao 266100,China)

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]青岛蔚蓝生物集团有限公司,山东青岛266100

出　　处：《食品与发酵工业》2024年第21期45-50,共6页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2021YFC2100205)。

摘　　要：为了进一步提高毕赤酵母发酵生产植酸酶的产量和原材料利用率,该文在毕赤酵母生产植酸酶过程中引入在线活细胞传感仪,在验证电容值与活菌浓度呈良好线性相关的基础上,通过在线活细胞仪反映的电容值、电导率值并结合氧消耗速率研究了营养盐添加量和诱导阶段甲醇流加速率对植酸酶产量的影响。结果表明,在营养盐添加量75%、甲醇最大流加速率1∶10(占空比)的工艺条件下,发酵终点植酸酶的最大酶活力和总酶活力分别达到16900 U/mL和853.39×10^(6) U,比优化前提高了29.8%和42.6%。In order to further enhance the production yield of phytase by Pichia pastoris and improve the utilization efficiency of raw materials,the online biomass monitoring technology was introduced to the study of the fermentation process.After validating the excellent linear correlation between capacitance values and viable cell concentration,this study utilized capacitance values and conductivity values reflected by the online biomass sensor,and the physiological parameter oxygen uptake rate,to investigate the impact of nutrient salt content and methanol feeding rates during the induction phase on phytase production.The results indicated that under the optimized conditions of 75%nutrient salt content and a maximum methanol feeding rate of 1∶10(duty cycle),the production titer and total activity of phytase at the end of fermentation reached 16900 U/mL and 853.39×10^(6) U,respectively,representing 29.8%and 42.6%increases compared to the unoptimized conditions.

关 键 词：植酸酶 毕赤酵母 在线活细胞仪 电容值 过程优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]