环介导等温扩增技术在食品安全检测领域的研究进展  

Application of loop-mediated isothermal amplification in the detection of food safety

作　　者：安红玉 陶泽 陈晨 卢增慧 万世园 李拖平 李苏红[1] AN Hongyu;TAO Ze;CHEN Chen;LU Zenghui;WAN Shiyuan;LI Tuoping;LI Suhong(College of Food Science,Shenyang Agricultural University,Shenyang 110886,China;Central Grain Reserve Xinmin Direct Warehouse Co.Ltd.,Xinmin 110300,China;State Grain Reserves Xuxinzhuang(Beijing)Depot Co.Ltd.,Beijing 101119,China)

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]中央储备粮新民直属库有限公司,辽宁新民110300 [3]中储粮(北京)徐辛庄直属库有限公司,北京101119

出　　处：《食品与发酵工业》2024年第21期388-396,共9页Food and Fermentation Industries

基　　金：沈阳市科技局社会治理科技专项(公共安全项目)(22322339)。

摘　　要：食品安全是一项重大而持久的挑战,对人类生活质量有着深远的影响。环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)是一种高效、灵敏、快捷、高特异性的核酸等温扩增技术。相较于传统核酸扩增方法,该技术针对目标DNA链上的6个区段,设计4个不同的引物。只需将样品DNA、引物、链置换型DNA聚合酶、dNTPs、Mg^(2+)等共同置于60~65℃反应30~60 min,经过一个步骤即可完成核酸快速扩增。广泛应用于食品安全检测领域中食源性致病菌污染、食品掺假、转基因食品、食品过敏原的检测。该文就LAMP技术的原理、与PCR技术的主要特点比较、现存问题以及在食品安全检测领域的创新应用进行了综述,为今后其在食品安全检测方面的应用推广提供参考。Food safety is a major and enduring challenge with far-reaching implications for the quality of human life.Loop-mediated isothermal amplification is an efficient,sensitive,fast and highly specific technique for nucleic acid isothermal amplification.Compared with traditional nucleic acid amplification methods,it designs 4 different primers for 6 regions on the target DNA strand.Sample DNA,primers,Bst DNA polymerase,dNTPs,Mg^(2+),etc.are added to the reaction tube.Nucleic acid amplification is performed at 60-65℃for 30-60 min and can be accomplished quickly in a single step.It is widely used in food safety testing to detect the presence of foodborne pathogenic bacteria contamination,food adulteration,genetically modified food,and food allergens.In this paper,the principle of LAMP technology,the comparison of the main features with PCR technology,the existing problems and the innovative applications in the field of food safety detection are reviewed to provide a reference for its application and promotion in the future.

关 键 词：环介导等温扩增 核酸检测 食品安全 结果判定 多重LAMP 

分 类 号：TS207.3[轻工技术与工程—食品科学] TS201.6[轻工技术与工程—食品科学与工程]