基于SYBR Green的实时qPCR法定量检测动物脂肪中的RNA  

Real Time qPCR Based on SYBR Green for Quantitative Detection of RNA in Animal Fat

作　　者：李德月 张玉梅 江磊[2] LI Deyue;ZHANG Yumei;JIANG Lei(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810001,China;Key Laboratory of Tibetan Medicine Research,Northwest Plateau Biology Institute,Chinese Academy of Sciences,Xining 810001,China)

机构地区：[1]青海大学农牧学院,青海西宁810001 [2]中国科学院西北高原生物研究所藏医药研究重点实验室,青海西宁810001

出　　处：《食品安全导刊》2024年第32期87-90,95,共5页China Food Safety Magazine

基　　金：青海省自然科学基金(2021-ZJ-924)。

摘　　要：建立一种从食用动物脂肪中提取RNA,并采用基于SYBR Green的实时荧光定量PCR(Quantitative Real-Time PCR,RT-qPCR)测定不同动物脂肪中RNA表达水平的方法。结果表明,改进后的TRIzol^((R))提取方法能够从动物脂肪中有效提取出200~500 ng·μL^(-1)的高纯度RNA。经RT-qPCR反应后所有提取的RNA样本均展现出了优异的扩增性能。本研究开发的TRIzol^((R))提取法结合RT-qPCR的新方法,可为后续动物脂肪的鉴别和追溯提供数据支撑。This article establishes a method for extracting RNA from edible animal fats and using SYBR Green based real-time fluorescent quantitative PCR(RT-qPCR)to determine RNA expression levels in different animal fats.The results indicate that the improved extraction method TRIzol^((R)) can effectively extract high-purity RNA of 200 ng·μL^(-1) to 500 ng·μL^(-1) from animal fat.After RT qPCR reaction,all extracted RNA samples exhibited excellent amplification performance.The extraction method TRIzol^((R)) developed in this study,combined with RT-qPCR,can provide data support for the identification and tracing of animal fat in the future.

关 键 词：动物脂肪 RNA提取 实时荧光定量PCR 定量检测 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]