基于非靶向代谢组学揭示灭活处理对植物乳植杆菌代谢物的影响  

Effect of inactivation treatment on metabolites of Lactiplantibacillus plantarum based on ultra high performance liquid chromatography orbitrap exploris mass spectrometry

作　　者：焦杨波 郑帅莉 赵洁[1] JIAO Yangbo;ZHENG Shuaili;ZHAO Jie(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学,乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古乳品生物技术与工程重点实验室,内蒙古呼和浩特010018

出　　处：《食品与发酵工业》2024年第22期131-136,共6页Food and Fermentation Industries

基　　金：内蒙古自治区直属高校基本科研业务费项目(BR230406);国家自然科学基金地区科学基金项目(32260573);内蒙古自治区科技计划项目(2020GG0101)。

摘　　要：随着对益生菌的深入研究,研究人员发现某些灭活菌体及其代谢产物也展现出一定的应用潜力。该研究以植物乳植杆菌为研究对象,采用超高效液相色谱高分辨质谱非靶向代谢组学技术并结合正交偏最小二乘法判别分析多元统计分析方法,探究灭活处理和未灭活处理植物乳植杆菌代谢物质的差异。结果表明,植物乳植杆菌灭活后共发现了24个潜在的差异代谢物(变量投影重要度>1,P<0.05,差异改变倍数>1.2或<0.8)。与不灭活处理相比,灭活处理后有19种上调的差异代谢物。KEGG富集分析表明,这些差异代谢物富集到的代谢通路有6条,差异极显著(P<0.01)的代谢通路是精氨酸和脯氨酸代谢,富集到这条代谢通路的差异代谢物有L-精氨酸和4-乙酰氨基丁酸酯。这些物质作为益生物质对于促进宿主健康、提高动物的生产性能、临床医疗领域的应用具有重要前景。该研究从代谢组学角度初步揭示了灭活前、后植物乳植杆菌代谢产物的差异性,为后续植物乳植杆菌的应用提供理论依据。With the in-depth study of probiotics,researchers have found that some inactivated bacteria and their metabolites also have certain application potential.In this study,ultra high performance liquid chromatography orbitrap exploris mass spectrometry(UHPLC-OE-MS)combined with orthogonal partial least squares discriminant analysis(OPLS-DA)were used to evaluate the metabolite composition of Lactiplantibacillus plantarum suspension with and without inactivation.Results showed that 24 potential differential metabolites were identified(variable importance in projection>1,P<0.05,fold change>1.2 or<0.8),in which 19 metabolites were up-regulated in inactivation group.Further,those differential metabolites were enriched in 6 metabolic pathways by analysis of Kyoto Encyclopedia of Genes and Genomes.L-arginine and proline metabolism pathways were extremely significant differences(P<0.01)between two groups,and L-arginine and 4-acetamidobutanoate were enriched in those two metabolic pathways.The application of these metabolites had important prospects for promoting host health,improving animal production performance,and clinical medical field.This study preliminarily revealed the differences in metabolites of Lactiplantibacillus plantarum before and after inactivation by metabolomics,and provided a theoretical basis for the subsequent application of Lactiplantibacillus plantarum.

关 键 词：植物乳植杆菌 代谢组学 灭活处理 差异代谢产物 离心上清液 

分 类 号：TS201.3[轻工技术与工程—食品科学]