QuEChERS-高效液相色谱串联质谱法测定猪肉中氟苯尼考残留量  

Determination of Florfenicol Residues in Pork by QuEChERS-HPLC-MS/MS

作　　者：罗嘉儿 欧佳灵 LUO Jiaer;OU Jialing(Guangdong Huizhou Quality and Measuring Supervision Testing Institute,Huizhou 516000,China)

机构地区：[1]广东省惠州市质量计量监督检测所,广东惠州516000

出　　处：《食品安全导刊》2024年第31期84-87,共4页China Food Safety Magazine

摘　　要：目的:建立QuEChERS净化结合高效液相色谱串联质谱法测定猪肉中氟苯尼考残留量的分析方法。方法:样品经捣碎均质化后用乙腈提取,提取液经QuEChERS净化,使用C18柱分离,以乙腈和超纯水为流动相进行洗脱,采用全扫描、多反应监测模式检测,内标法定量。结果:在0~50μg·L-1浓度范围内氟苯尼考具有良好的线性关系,相关系数R2≥0.999,定量限为0.02μg·kg^(-1),检出限为0.01μg·kg^(-1);在10μg·kg^(-1)、20μg·kg^(-1)、50μg·kg^(-1)3个添加浓度水平下,加标回收率为98.2%~108.5%,RSD为3.24%~5.75%。结论:该方法前处理操作简单,准确度和灵敏度高,满足猪肉中氟苯尼考残留量的检测要求。Objective:To establish a method for the determination of florfenicol residue in pork by QuEChERS cleanup combined with high performance liquid chromatography tandem mass spectrometry.Method:The sample was crushed and homogenized and then extracted with acetonitrile.The extract was cleaned up by QuEChERS and separated by C18 column.Acetonitrile and ultrapure water were used as mobile phases for elution.Full scan and multiple reaction monitoring mode were used for detection and internal standard method for quantification.Result:Florfenicol had a good linear relationship in the concentration range of 0~50μg·L-1,with correlation coefficient R2≥0.999,limit of quantification of 0.02μg·kg^(-1),and limit of detection of 0.01μg·kg^(-1).At the three spiked concentration levels of 10μg·kg^(-1),20μg·kg^(-1),and 50μg·kg^(-1),the recoveries were 98.2%~108.5%,and the RSD were 3.24%~5.75%.Conclusion:This method has simple pretreatment operation,high accuracy and sensitivity,and meets the requirements for the detection of florfenicol residues in pork.

关 键 词：氟苯尼考 QUECHERS 高效液相色谱串联质谱法 猪肉 

分 类 号：O657.63[理学—分析化学] TS251.51[理学—化学]