保健食品中红景天苷和酪醇含量的测量不确定度评定  

Evaluation of Uncertainty in the Determination of Salidroside and Tyrosol in Health-Care Food

作　　者：谢芳云 李永强 袁靖 冉丹 崔晓娇 夏炜杰 XIE Fangyun;LI Yongqiang;YUAN Jing;RAN Dan;CUI Xiaojiao;XIA Weijie(Changsha Institute for Food and Drug Control,Changsha 410036;National Alcohol Products Quality Inspection andTesting Center(Hunan),Changsha 410036;Changsha County for Comprehensive Testing Center,Changsha 410199)

机构地区：[1]长沙市食品药品检验所,长沙410036 [2]国家酒类产品质量检验检测中心(湖南),长沙410036 [3]长沙县综合检测中心,长沙410199

出　　处：《食品工业》2024年第10期303-308,共6页The Food Industry

基　　金：湖南省市场监督管理局科技项目(2024KJJH34)。

摘　　要：依据2020年版《保健食品理化及卫生指标检验与评价技术指导原则》对保健食品红景天胶囊中红景天苷和酪醇的测定进行不确定度评定。建立高效液相色谱法(HPLC)测定红景天胶囊中红景天苷与酪醇含量的不确定度数学模型,同时对检测流程中可能产生不确定度的各分量进行评定。结果显示,在95%置信概率(k=2)下,红景天胶囊中红景天苷和酪醇含量的扩展不确定度分别为0.12 mg/g和0.013 mg/g,检测结果为X红景天苷=(3.67±0.12)mg/g,X酪醇=(0.400±0.013)mg/g。经评定,该模型的不确定度主要来源于标准物质的纯度、标准系列溶液配制和样品回收率。Evaluate the uncertainty of the determination of salidroside and tyrosol in health-care food rhodiola capsules based on 2020 Edition of the Technical Guiding Principles for Inspection and Evaluation of Physicochemical and Hygienic Indicators of Health Food.A mathematical model for the uncertainty of determining the content of salidroside and tyrosol in Rhodiola capsules using high-performance liquid chromatography(HPLC)was established,and the components that may generate uncertainty during the detection process were evaluated.The results showed that at 95%confidence probability(k=2),the expansion uncertainty of salidroside and tyrosol in rhodiola capsules was 0.12 mg/g and 0.013 mg/g,respectively.The test results were espressed as:Xsalidroside=(3.67±0.12)mg/g,Xtyrosol=(0.400±0.013)mg/g.After evaluation,the uncertainty of the model mainly comes from the purity of standard substances,the preparation of standard series solutions and the sample recovery.

关 键 词：高效液相色谱法 保健食品 红景天苷 酪醇 不确定度评定 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]