萌发芝麻提取物改善鼠伤寒沙门氏菌感染小鼠焦虑和肠道炎症  

Germinated Sesame Extract Improving Anxiety and Gut Inflammation in Salmonella Typhimurium-infected Mice

作　　者：张滋龙 杨雅娴 李竞萌 李菁 王玉堂[2] 原田 郭瑞[2] 刘志刚[2] ZHANG Zi-long;YANG Ya-xian;LI Jing-meng;LI Jing;WANG Yu-tang;YUAN Tian;GUO Rui;LIU Zhi-gang(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao,Shandong 266109,China;College of Food Science and Engineering,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China;College of Chemistry and Pharmacy,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [3]西北农林科技大学化学与药学院,陕西杨凌712100

出　　处：《粮油食品科技》2024年第6期151-160,共10页Science and Technology of Cereals,Oils and Foods

基　　金：国家自然科学基金青年科学基金项目(82103842);中国博士后科学基金会(2022M72261)。

摘　　要：芝麻是一种在我国被广泛种植的作物,它含有大量的脂肪和蛋白质,还有膳食纤维、芝麻木酚素及γ-氨基丁酸(GABA)等活性营养成分。近几年很多研究指出,GABA和芝麻木酚素中的芝麻酚具有缓解焦虑、抑制炎症的作用,但其在食源性致病菌侵染过程中的具体作用及机制尚未报道。对乙醇浸提萌发芝麻得到的萌发芝麻提取物进行检测,发现与芝麻相比芝麻酚和GABA含量分别提升6倍和4倍。使用鼠伤寒沙门氏菌SL1344侵染小鼠构建动物模型,探究萌发芝麻提取物、芝麻酚和GABA对鼠伤寒沙门氏菌感染小鼠的影响,发现萌发芝麻提取物可以减少沙门氏菌感染小鼠的体重降低、改善焦虑行为、减少对小鼠脾脏的侵染、增加感染小鼠结肠绒毛长度和黏蛋白含量、抑制结肠炎症因子、增加粪便中乙酸和丙酸含量。萌发芝麻提取物缓解了沙门氏菌感染小鼠的焦虑,同时对小鼠的肠道具有保护作用。本研究为饮食干预缓解焦虑、保护肠道提供相关理论参考。Sesame is a widely cultivated crop in China,which contains a large amount of fat and protein,as well as dietary fiber,sesame lignans,γ-Aminobutyric acid(GABA)and other active nutrients.In recent years,many studies have pointed out that GABA and sesamol from sesamin could possess anxiolytic and anti-inflammatory properties,but their specific roles and mechanisms during foodborne pathogenic bacterial infections remain unexplored.We analyzed the extract obtained from ethanol-soaked germinated sesame and found that the contents of sesamol and GABA were 6 times and 4 times higher,respectively,compared to non-germinated sesame.Constructing an animal model by infecting mice with Salmonella typhimurium SL1344,and exploring the effects of germinating sesame extract,sesamol,and GABA on Salmonella typhimurium infection in mice.It was found that germinating sesame extract could reduce weight loss in Salmonella infected mice,and improve anxiety behavior in Salmonella infected mice.Germinating sesame extract also reduced Salmonella infection in mouse spleen,and increased colon villus length and mucin content in Salmonella infected mice,which could inhibit colitis factors,and increase acetic acid and propionic acid content in feces.Germinated sesame extract alleviated anxiety and protected the gut in mice infected with Salmonella.This study was aimed to provide a theoretical reference for dietary intervention to alleviate anxiety and protect the gut.

关 键 词：芝麻 萌发 萌发芝麻提取物 鼠伤寒沙门氏菌 焦虑 肠道炎症 

分 类 号：TS201.4[轻工技术与工程—食品科学]