黑果枸杞果实多糖的超声辅助双水相萃取工艺优化、单糖组成分析及胰脂肪酶抑制活性评价  

Optimization of the Ultrasonic-assisted Aqueous Two-phase Extraction of Lycium ruthenicum Polysaccharides and Its Monosaccharide Composition and Pancreatic Lipase Inhibitory Activity

作　　者：谭婉 卫阳飞[1] 李枫林 鲁宁 龚永鹏 李全春 章毅 TAN Wan;WEI Yangfei;LI Fenglin;LU Ning;GONG Yongpeng;LI Quanchun;ZHANG Yi(Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Hexi University,Zhangye 734000,China;College of Chemistry and Chemical Engineering,Hexi University,Zhangye 734000,China;Chinese Medicine Hospital of Minle County,Zhangye 734000,China)

机构地区：[1]河西学院甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [2]河西学院化学化工学院,甘肃张掖734000 [3]民乐县中医医院,甘肃张掖734000

出　　处：《食品工业科技》2024年第22期158-168,共11页Science and Technology of Food Industry

基　　金：国家级大学生创新训练项目(202310740003);河西学院博士科研启动项目(KYQD2023003);甘肃省自然科学基金(23JRRG0003);甘肃省高校青年博士支持项目(2023QB-081);甘肃省河西走廊特色资源利用重点实验室面上项目(XZ2301)。

摘　　要：目的:优化黑果枸杞果实多糖的制备工艺,分析单糖组成,并对其体外胰脂肪酶抑制活性进行评价。方法:采用超声辅助双水相萃取法,以多糖回收率和提取率的总评值为评价指标,在单因素实验基础上,通过响应面试验优化黑果枸杞果实多糖提取工艺;采用柱前衍生化高效液相色谱法研究其单糖组成;通过棕榈酸对硝基苯酯法测定黑果枸杞果实多糖的体外胰脂肪酶抑制活性。结果:黑果枸杞果实多糖的最佳提取工艺为:乙醇-硫酸铵体系,乙醇质量分数30.89%,硫酸铵质量分数17.00%,提取温度49.05℃,提取时间81.85 min,液料比29.80 g/g,预测总评值为0.9242,多糖提取率为104.76 mg/g,回收率为96.96%;黑果枸杞果实多糖由甘露糖、核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成,相对摩尔比分别为4.92:0.29:4.80:0.40:9.69:44.79:17.04:18.06;黑果枸杞果实多糖抑制胰脂肪酶活力的IC_(50)值为1.51 mg/mL。结论:响应面试验优化得到的黑果枸杞果实多糖制备工艺稳定,单糖组成检测方法准确可靠,制备所得多糖具有良好的体外胰脂肪酶抑制活性,为黑果枸杞果实多糖的进一步开发利用提供了依据。Objective:To optimize the preparation process of polysaccharides from Lycium ruthenicum,analyze the monosaccharide composition and evaluate its pancreatic lipase inhibitory activity in vitro.Methods:Ultrasound-assisted aqueous two-phase extraction method was employed.Taking the overall evaluation value of polysaccharide recovery and extraction rate as the evaluation index,on the basis of single factor experiment,response surface analysis was conducted to optimize the extraction process.The composition of monosaccharides was studied by high performance liquid chromatography with pre-column derivatization.The pancreatic lipase inhibitory activity of Lycium ruthenicum polysaccharides was evaluated by p-nitrophenyl palmitate method.Results:The optimum extraction conditions:Ethanol-ammonium sulfate system,ethanol concentration 30.89%,ammonium sulfate concentration 17.00%,extraction temperature 49.05℃,extraction time 81.85 min,liquid to material ratio 29.80 g/g.At this time,the predicted total evaluation value was 0.9242,and the extraction and recovery rates were 104.76 mg/g and 96.96%,respectively.Lycium ruthenicum polysaccharides were composed of monosaccharides such as mannose,ribose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and arabinose,with the relative molar ratios of 4.92:0.29:4.80:0.40:9.69:44.79:17.04:18.06,respectively.The IC_(50) value of Lycium ruthenicum polysaccharides inhibiting pancreatic lipase was 1.51 mg/mL.Conclusion:The preparation process of Lycium ruthenicum polysaccharides optimized by response surface methodology was stable.The detection method of monosaccharide composition was accurate and reliable.Lycium ruthenicum polysaccharides had good pancreatic lipase inhibitory activity in vitro.The results would provide a basis for the further development and utilization of Lycium ruthenicum polysaccharides.

关 键 词：黑果枸杞 多糖 双水相萃取 响应面试验 单糖组成 胰脂肪酶 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]