蒲公英红枣汁对D-半乳糖诱导大鼠肝损伤保护作用  

Protective Effect of Dandelion Jujube Juice on D-galactose-induced Liver Injury in Rats

作　　者：尤佳伟 韩如意 陈月晓 陈芬 刘媛 李明月 李红英 李奕阳 孙亚凤 田粟[4,5] Jiawei;HAN Ruyi;CHEN Yuexiao;CHEN Fen;LIU Yuan;LI Mingyue;LI Hongying;LI Yiyang;SUN Yafeng;TIAN Su(Department of Clinical Nutrition,The First Affiliated Hospital of Xi'an Medical University,Xi'an 710077,China;Tongzhou Center for Disease Control and Prevention,Beijing 101100,China;Institute of Biotechnology and Health,Beijing Academy of Science and Technology,Beijing 100089,China;School of Public Health,Hebei Medical University,Shijiazhuang 050017,China;Hebei Key Laboratory of Environment and Human Health,Shijiazhuang 050017,China)

机构地区：[1]西安医学院第一附属医院临床营养科,陕西西安710077 [2]北京市通州区疾病预防控制中心,北京101100 [3]北京市科学技术研究院生物技术与健康研究所,北京100089 [4]河北医科大学公共卫生学院,河北石家庄050017 [5]河北省环境与人群健康重点实验室,河北石家庄050017

出　　处：《食品工业科技》2024年第22期331-337,共7页Science and Technology of Food Industry

基　　金：河北省自然科学基金(H2019423087)。

摘　　要：目的:探讨蒲公英红枣汁对D-半乳糖诱导肝损伤大鼠的保护作用及可能的作用机制。方法:采用持续腹腔注射500 mg/kg D-半乳糖水溶液6周,建立慢性肝损伤模型。将肝损伤大鼠随机分为模型组和蒲公英红枣汁组(10 mL/kg·d),灌胃28 d后,计算肝脏指数、HE染色和油红染色观察肝组织病理变化;试剂盒法测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST),和肝组织丙二醛(MDA)、过氧化氢酶(CAT)水平;蛋白质免疫印迹法测定肝脏组织Kelch样环氧氯丙烷相关蛋白1(Keap1)、核因子E2相关因2(Nrf2)、血红素加氧酶1(HO-1)、BCL2-Associated X蛋白(Bax)、B淋巴细胞瘤-2基因(Bcl-2)蛋白表达。结果:与空白组比较,模型组肝脏脏器指数显著升高(P<0.05),肝组织细胞出现凋亡,脂质沉积面积增加,同时血清ALT(P<0.05)、AST(P<0.01)、肝组织MDA水平(P<0.01)、Keap1蛋白(P<0.01)、Bax蛋白(P<0.01)表达显著增加,肝组织CAT水平(P<0.01)、Nrf2(P<0.01)、HO-1(P<0.05)、Bcl-2(P<0.01)蛋白表达量显著降低,提示肝脏功能受损。与模型组相比,蒲公英红枣汁干预显著降低了脏器指数(P<0.05)、血清中ALT(P<0.05)、AST活性(P<0.05)、肝组织MDA含量(P<0.05)、Keap1蛋白表达(P<0.01),同时升高了肝组织CAT活性(P<0.05)、Nrf2蛋白(P<0.05)、HO-1蛋白(P<0.05)、Bcl-2蛋白的表达量(P<0.01),且肝组织形态明显改善,细胞凋亡数量和脂质沉积面积明显减少。结论:蒲公英红枣汁可能具有一定保护D-半乳糖诱导的肝损伤的作用,这可能与激活Keap1/Nrf2/HO-1信号通路,提高肝脏抗氧化能力和抑制肝细胞凋亡有关。Objective:To evaluate the protective effect and possible mechanism of dandelion jujube juice on D-galactose-induced liver injury in Sprague Dawley(SD)rats.Methods:D-galactose was intraperitoneally injected into the rats at a dose of 500 mg/kg for six weeks to establish a chronic liver injury model.These rats treated with D-galactose were then randomly divided into model group and dandelion jujube juice group(treated with 10 mL/kg·d of dandelion jujube juice by intragastric administration for 28 d).The liver index was calculated,and the pathological changes in liver tissue were observed using HE staining and oil red O staining.Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels in serum,malondialdehyde(MDA)content and catalase(CAT)activity in liver tissue were determined by corresponding assay kit.Western blot analysis was used to detect the protein expression,including Kelch like epichlorohydrin associated protein 1(Keap1),nuclear factor E2 related factor 2(Nrf2),heme oxygenase-1(HO-1),BCL2 associated X(Bax),and B-cell lysoma-2(Bcl-2)proteins in liver tissue.Results:Compared with the blank group,the liver index and liver lipid deposition area were increased in the model group.In addition,the model group showed a significant increase in the levels of serum ALT(P<0.05),AST(P<0.01),liver tissue MDA(P<0.01),Keap1(P<0.01),Bax(P<0.01)protein expression,as well as a significant decrease in liver tissue CAT activity(P<0.01),Nrf2(P<0.01),HO-1(P<0.05),and Bcl-2(P<0.01)protein expression levels,these indicate liver damage.Furthermore,in comparison with the model group,dandelion jujube juice could significantly reduce liver index(P<0.05),the levels of serum ALT(P<0.05)and AST(P<0.05),liver tissue MDA(P<0.05),Keap1(P<0.01)protein expression,while CAT activity(P<0.05),Nrf2(P<0.05),HO-1(P<0.05)and Bcl-2(P<0.01)expression in liver tissue were significantly increased.Dandelion jujube juice also significantly improved the morphology of liver tissue and reduced the number of liver cell apoptosis and the area of

关 键 词：蒲公英红枣汁 D-半乳糖 肝损伤 凋亡 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]