姑辽茶粗多糖提取工艺优化及其免疫调节活性评价  

Optimization of the Extraction Process of Crude Polysaccharide from Guliao Tea and Evaluation of Its Immunomodulatory Activity

作　　者：罗雨菲 黄秀壁 苏芬安 谭义秋[1] 陈永[1,2] 刘小玲 LUO Yufei;HUANG Xiubi;SU Fen'an;TAN Yiqiu;CHEN Yong;LIU Xiaoling(School of Chemical and Biological Engineering,Guangxi Minzu Normal University,Chongzuo 532200,China;School of Food Science and Technology,South China University of Technology,Guangzhou 510640,China;Graduate School,Guangxi University,Nanning 530004,China)

机构地区：[1]广西民族师范学院化学与生物工程学院,广西崇左532200 [2]华南理工大学食品科学与工程学院,广东广州510640 [3]广西大学研究生院,广西南宁530004

出　　处：《食品工业科技》2024年第23期161-169,共9页Science and Technology of Food Industry

基　　金：广西民族师范学院国家级大学生创新创业训练计划项目(202310604026,202410604049);2024年度广西高校中青年教师科研基础能力提升项目(2024KY0788);广西大学对口支援学科建设项目(2023M03);广西民族师范学院2022年度高层次人才项目(2022GCC001)。

摘　　要：为探究姑辽茶粗多糖的最佳提取工艺并对其免疫活性进行评价。以姑辽茶为原料,固定超声功率400 W,在探究超声时间、提取温度、提取次数、料液比对姑辽茶粗多糖得率的影响的基础上,通过Box-Behnken响应面法对提取工艺参数进行优化;采用CCK-8法检测姑辽茶粗多糖对RAW264.7小鼠巨噬细胞增殖活性的影响,测定TNF-α、IL-6和NO的分泌水平以评价其免疫调节活性。结果表明:在超声时间32 min、提取温度64℃、提取4次、料液比1:44 g/mL的工艺条件下提取的姑辽茶粗多糖得率最高,为13.12%±0.11%;姑辽茶粗多糖中的总糖含量为61.8%±0.09%。姑辽茶粗多糖对RAW264.7小鼠巨噬细胞增殖活性影响作用微弱,基本无毒性作用,在50~400μg/mL范围内对促进RAW264.7小鼠巨噬细胞分泌TNF-α、IL-6和NO呈现一定的量效关系。通过对姑辽茶粗多糖的最佳提取工艺优化及评价其免疫活性,为更好地挖掘姑辽茶多糖资源提供科学依据。In order to investigate the optimal extraction process of crude polysaccharide from Guliao tea and evaluate its immunomodulatory activity,Guliao tea as the raw material and fixed the ultrasonic power at 400 W,the effects of parameters including ultrasonic time,extraction temperature,extraction times and solid-liquid ratio on the yield of Guliao tea crude polysaccharide were examined.The parameters were optimized by Box-Behnken response surface design.The impact of Guliao tea crude polysaccharide on the proliferation activity of RAW264.7 murine macrophages was detected by CCK-8 assay.The secretions of TNF-α,IL-6 and NO were measured to evaluate immunomodulatory activity.The results indicated that the highest yield of Guliao tea crude polysaccharide was 13.12%±0.11%under the conditions of 32 min of ultrasonic time,64℃of extraction temperature,four extraction circles and a 1:44 g/mL solid-liquid ratio.The total sugar amount in the crude polysaccharide from Guliao tea was 61.8%±0.09%.Guliao tea crude polysaccharide exhibited a slight effect on the proliferation activity of RAW264.7 murine macrophages with essentially no toxicity and demonstrated a doseresponse relationship in promoting the secretion of TNF-α,IL-6,and NO within the range of 50~400μg/mL.By optimizing the extraction process of crude polysaccharide from Guliao tea and evaluating its immunomodulatory activity,the study provided a scientific basis for more effective exploration and utilization of Guliao tea polysaccharide.

关 键 词：姑辽茶 多糖 响应面优化 小鼠巨噬细胞 免疫活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]