罗非鱼头硫酸软骨素对RAW 264.7细胞和小鼠免疫刺激活性研究  

Immunostimulatory Activity of Tilapia-head Chondroitin Sulfate in RAW 264.7 Cells and Mice

作　　者：黄后培 袁媛 汪卓 陈菁 宋兵兵 钟赛意 HUANG Houpei;YUAN Yuan;WANG Zhuo;CHEN Jing;SONG Bingbing;ZHONG Saiyi(Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Engineering Technology Research Center of Seafood,College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Institute,Guangdong Ocean University,Shenzhen 518063,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518063 [3]海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《食品工业科技》2024年第23期381-387,共7页Science and Technology of Food Industry

基　　金：国家重点研发计划(2020YFD0901101);广东省重点领域研发计划资助(2020B1111030004);广东省高校科技创新团队项目(2021KCXTD021)。

摘　　要：本文采用了RAW 264.7细胞和免疫抑制模型,通过体外、体内实验研究罗非鱼头硫酸软骨素(CS)的免疫刺激活性。结果表明,罗非鱼头硫酸软骨素(CS)可以诱导RAW 264.7细胞产生NO、IL-1β、IL-6、IL-10、TNF-α;环磷酰胺(CTX)诱导小鼠的胸腺萎缩(胸腺指数0.11%±0.01%),低剂量CS(胸腺指数(0.17%±0.03%))、高剂量CS(胸腺指数(0.18%±0.02%))干预后,显著上调了小鼠胸腺指数(P<0.05);同时,CS干预显著上调CTX诱导小鼠脾脏指数(P<0.05),上调绒毛长度和V/C值并且高剂量CS显著上调了绒毛长度(330.92±21.55)μm(P<0.05);另外,CS对CTX诱导小鼠血清sIgA的分泌有促进作用。这些结果表明CS可以改善免疫抑制小鼠肠道屏障损伤及免疫功能。因此,本研究可为罗非鱼硫酸软骨素的肠道黏膜免疫调节作用提供理论基础,并表明罗非鱼头硫酸软骨素(CS)是一种潜在的可替代鲨鱼硫酸软骨素的免疫调节多糖,并可能成为干预免疫疾病相关功能食品的潜在材料。In this study,RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of tilapia-head chondroitin sulfate(CS)through in vitro and in vivo experiments.The results showed that tilapiahead chondroitin sulfate(CS)could induce the produce of NO,IL-1β,IL-6,IL-10 and TNF-αin RAW 264.7 cells.Cyclophosphamide(CTX)induced thymic atrophy in mice(thymic index 0.11%±0.01%),while thymic index was significantly up-regulated by low-dose CS(thymic index(0.17%±0.03%))and high-dose CS(thymic index(0.18%±0.02%))interventions(P<0.05).Meanwhile,CS significantly increased spleen index in CTX-induced mice(P<0.05),promoted villus length and V/C value,with high-dose CS significantly increased villus length(330.92±21.55)μm(P<0.05).Furthermore,CS enhanced the secretion of serum sIgA in CTX-induced mice,indicating that CS could improve intestinal barrier damage and immune function in immunosuppressive mice.Therefore,this study provides a theoretical basis for the intestinal mucosal immune regulation of tilapia chondroitin sulfate,and suggests that tilapia-head chondroitin sulfate(CS)is a potential immunoregulatory polysaccharide that could replace shark chondroitin sulfate,potentially serving as a promising material for functional foods aimed at intervening in immune-related diseases.

关 键 词：罗非鱼 硫酸软骨素 免疫刺激活性 免疫抑制 肠道屏障 RAW 264.7 

分 类 号：TS201.4[轻工技术与工程—食品科学]