Integrated photothermal microcarriers for precise exosome-secreted microRNA profiling in breast cancer diagnosis  

作　　者：Yunjie Shi Yun Cheng Peiyu Chen Lexiang Zhang Fangfu Ye 

机构地区：[1]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China [2]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,China [3]Department of Pediatrics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China [4]Joint Centre of Translational Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China [5]Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing,China

出　　处：《Quantitative Biology》2024年第4期389-399,共11页定量生物学（英文版）

基　　金：National Key Research and Development Program of China,Grant/Award Number:2022YFA1405002;National Natural Science Foundation of China,Grant/Award Numbers:12325405,22307098;Wenzhou Institute,University of Chinese Academy of Sciences,Grant/Award Number:WIUCASQD2021012;Wenzhou high‐level innovation team;Development and application team of functional liv;Key Projects of Wenzhou Science and Technology Bureau,Grant/Award Number:ZG2023013;Wenzhou Basic Research Projects,Grant/Award Number:Y2023147;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Number:LGF22C100003;Key Laboratory of Structural Malformations in Children of Zhejiang Province,Grant/Award Number:ZJET2301Z。

摘　　要：Breast cancer constitutes a significant global health burden,while conventional diagnosis approaches may lack precision and can be discomforting for patients.Exosomes have emerged as promising biomarkers for breast cancer due to their participation in diverse pathological processes,and a convenient analysis platform is believed to greatly promote its application.In this study,we propose a novel digital PCR approach utilizing near-infrared(NIR)photo-responsive thermosensitive microcarriers integrated with black phosphorus for quantifying microRNA(miRNA)biomarkers within exosomes.Petal-like biomimetic nanomaterials were firstly assembled for nonspecific exosome capture based on the affinity effect of avidin and biotin.Photothermal-responsive microcarriers,fabricated using gelatin-based substrates blended with photothermal nanocomposite,exhibited NIRinduced heating and reversible phase transition properties.We optimized synthesis parameters on thermal response and established a programmable and controllable NIR light source module.The results indicated a significant elevation in the levels of biomarkers miRNA-1246 and miRNA-122,with fold increases ranging from 6.2 to 23.6 and 5.9 to 13.0,respectively,in breast cancer cell lines MCF-7 and MDA-MB-231 compared to healthy control cells HUVEC.This study offers broad prospects for utilizing exosomes to resolve predictive biomarkers.

关 键 词：breast cancer digital PCR EXOSOMES MICRORNA PHOTOTHERMAL 

分 类 号：R737.9[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]