Phenylboronic-tannin nanocolloids that scavenge subchondral reactive oxygen microenvironment and inhibit RANKL induced osteoclastogenesis for osteoarthritis treatment  

作　　者：Xiaoqun Li Yufang Kou Jia Jia Minchao Liu Runze Gao Yuhong Li Gang Li Shuogui Xu Wei Song Yang Xie Xiaomin Li Tiancong Zhao 

机构地区：[1]School of Chemistry and Materials, Department of Chemistry, Laboratory of Advanced Materials and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, State Key Laboratory of Molecular Engineering of Polymers, Collaborative Innovation Center of Chemistry for Energy Materials (2011-iChEM), Fudan University, Shanghai, 200433, China [2]Department of Orthopaedics Trauma and Department of Ophthalmology, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China [3]State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, China

出　　处：《Nano Research》2024年第11期9898-9907,共10页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22305042(Tiancong Zhao),22075049(Xiaomin Li),21875043(Xiaomin Li),82201748(Xiaomin Li));the Fundamental Research Funds for the Central Universities(No.20720220010(Xiaomin Li));the Natural Science Foundation of Shanghai(No.22ZR1478900(Xiaomin Li));the Shanghai Rising-Star Program(Nos.20QA1401200(Xiaomin Li),22YF1402200(Tiancong Zhao),21YF1459200(Xiaomin Li));Shanghai Pilot Program for Basic Research-Fudan University(No.22TQ004);Young Elite Scientist Sponsorship Program by Chinese Chemical Society(Tiancong Zhao),Xiaomi Young Talents Program(Tiancong Zhao),Qatar Research Development and Innovation Council(No.ARG01-0602-230467).

摘　　要：The excessive reactive oxygen species (ROS) accumulation and overactivated osteoclastogenesis in subchondral bone has proved to be a major cause of osteoarthritis (OA). Scavenging of ROS microenvironment to inhibit the osteoclastogenesis is highly valued in the therapeutic process of osteoarthritis. Despite the excellent ability of polyphenolic colloidal to scavenge reactive oxygen species and its affinity for macrophages, the preparation of polyphenolic colloidal nanoparticles is limited by the complex intermolecular forces between phenol molecules and the lack of understanding of polymerization/sol-gel chemistry. Herein, our work introduces a novel poly-tannin-phenylboronic colloidal nanoparticle (PTA) exclusively linked by ROS-responsive bondings. Nanocolloidal PTA has a uniform particle size, is easy and scalable to synthesize, has excellent scavenging of ROS, and can be slowly degraded. For in vitro experiments, we demonstrated that, PTA could eliminate ROS within RAW264.7 cells and impede osteoclastogenesis and bone resorption. RNA sequencing results of PTA-treated RAW264.7 cells further reveal the promotion of antioxidant activity and inhibition of osteoclastogenesis. For in vivo experiments, PTA could eliminate the ROS environment and reduce the number of osteoclasts in the subchondral bone, thereby alleviating the damage of subchondral bone and symptoms of osteoarthritis. Our research, by delving into the formation of polyphenol colloidal nanoparticles and validating their role in ROS scavenging to inhibit osteoclastogenesis in subchondral bone, may open new avenues for OA treatment in the future.

关 键 词：POLYPHENOL nanoparticle reactive oxygen species(ROS) OSTEOCLASTOGENESIS subchondral bone 

分 类 号：O64[理学—物理化学]