藜蒿叶多糖提取工艺优化及其α-葡萄糖苷酶抑制活性和抗氧化活性评价  

Optimization of extraction process of polysaccharide from Artemisia selengensis Turcz leaf and evaluation on itsα-glucosidase inhibitory activity and antioxidant activity

作　　者：邹伟 丁翘 陶雪茹 韩敏 朱思莹 王思颖 袁涛 涂宗财[1,2,3] ZOU Wei;DING Qiao;TAO Xue-ru;HAN Min;ZHU Si-ying;WANG Si-ying;YUAN Tao;TU Zong-cai(National R&D Center for Freshwater Fish Processing,Jiangxi Normal University;College of Life Science,Jiangxi Normal University,Nanchang 330022,China;State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330027,China)

机构地区：[1]江西师范大学国家淡水鱼加工技术研发专业中心 [2]江西师范大学生命科学学院,南昌330022 [3]南昌大学食品科学与资源挖掘全国重点实验室,南昌330027

出　　处：《天然产物研究与开发》2024年第11期1900-1909,共10页Natural Product Research and Development

基　　金：国家自然科学基金地区项目(32260596);江西省自然科学基金青年基金(20232BAB215063);江西省教育厅科学技术研究项目(GJJ210320);南昌大学食品科学与资源挖掘全国重点实验室开放基金(SKLF-KF-202204)。

摘　　要：采用热水浸提法提取藜蒿叶多糖(polysaccharide from Artemisia selengensis Turcz leaf,ASLP),通过单因素试验探究提取温度、提取时间、提取次数、料液比4个因素对藜蒿叶粗多糖得率的影响;在此基础上,采用响应面法优化提取工艺。同时测定ASLP糖含量、糖醛酸、分子量分布、单糖组成、α-葡萄糖苷酶抑制活性和抗氧化活性。结果表明,藜蒿叶粗多糖的最佳提取工艺为:提取温度95℃、提取时间3 h、提取次数为3次、料液比为1∶30(g/mL),在此条件下藜蒿叶粗多糖得率为17.23%±0.26%。理化性质结果发现ASLP糖醛酸含量为31.70%±0.73%,高于中性糖含量(26.20%±1.56%),分子量分布结果表明ASLP为分布较广的非均一多糖。单糖组成结果显示半乳糖醛酸比例最高,为38.76%,其次是半乳糖(22.88%)和鼠李糖(10.92%)。此外,ASLP对DPPH自由基、ABTS+自由基和α-葡萄糖苷酶的半抑制浓度(IC50)分别为0.1208、0.01601、1.364 mg/mL,表明其具有良好的体外降血糖和抗氧化活性。The polysaccharide from Artemisia selengensis Turcz leaf(ASLP)were extracted by hot water extraction method.The effects of extraction temperature,extraction time,extraction times and solid-liquid ratio on the yield of crude ASLP were studied by single factor experiment,and the extraction process was optimized by response surface method based on the results of single factor experiment.Meanwhile,the amounts of neutral sugar and uronic acid,molecular weight distribution,monosaccharide composition,α-glucosidase inhibitory activity and antioxidant activity of ASLP were determined.The results showed that the optimum extraction process of crude ASLP was as follows:extraction temperature 95℃,extraction time 3 h,extraction times 3,solid-liquid ratio 1∶30(g/mL).The yield of crude ASLP was 17.23%±0.26%by the optimum extraction process.The results of physical and chemical properties study showed that the content of uronic acid of ASLP was 31.70%±0.73%,which was higher than the content of neutral sugar(26.20%±1.56%).The molecular weight determination indicated that ASLP was a widely distributed heterogeneous polysaccharide.It was found from the monosaccharide composition study that galacturonic acid had the highest proportion(which was 38.76%)in ASLP,followed by galactose(22.88%)and rhamnose(10.92%).Furthermore,IC 50 of ASLP on DPPH and ABTS+free radicals andα-glucosidase were 0.1208,0.01601 and 1.364 mg/mL,respectively,indicating that ASLP has good hypoglycemic and antioxidant activity in vitro.

关 键 词：藜蒿叶多糖 工艺优化 响应面法 Α-葡萄糖苷酶 抗氧化活性 

分 类 号：Q539[生物学—生物化学]