3D-printed tri-element-doped hydroxyapatite/polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration  

作　　者：Hao Huang Lei Qiang Minjie Fan Yihao Liu Anchun Yang Dongbiao Chang Jinsheng Li Tong Sun Yiwei Wang Ruoyi Guo Hanjie Zhuang Xiangyu Li Tailin Guo Jinwu Wang Huan Tan Pengfei Zheng Jie Weng 

机构地区：[1]Key Laboratory of Advanced Technologies of Materials(MOE),School of Materials Science and Engineering,College of Medicine,Southwest Jiaotong University,Chengdu,610031,PR China [2]Department of Orthopaedic Surgery,Children’s Hospital of Nanjing Medical University,Nanjing 210008,PR China [3]Shanghai Key Laboratory of Orthopedic Implant,Department of Orthopedic Surgery Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine Shanghai 200011,PR China [4]School of Mechanical and Electrical Engineering,Henan University of Technology,Zhengzhou 450001,PR China

出　　处：《Bioactive Materials》2024年第1期18-37,共20页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(52071277,21802009);Jiangsu Provincial Key Research and Development Program(CN)(BE2019608);China Postdoctoral Science Foundation(2022M721685);Jiangsu Health Commission Medical Research Program(2020158);National Facility for Translational Med-icine(Shanghai)Open Program(TMSK-2021-304);2020 Nanjing In-ternational Science and Technology Cooperation Program;Nanjing Medical Science and Technology Development Key Program(ZKX18041).

摘　　要：The resection of malignant osteosarcoma often results in large segmental bone defects,and the residual cells can facilitate recurrence.Consequently,the treatment of osteosarcoma is a major challenge in clinical practice.The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly,and repair the resultant bone defects as well as avoid bacterial infections.Herein,we fabricated a selenium/strontium/zinc-doped hydroxyapatite(Se/Sr/Zn-HA)powder by hydrothermal method,and then employed it with polycaprolactone(PCL)as ink to construct composite scaffolds through 3D printing,and finally introduced them in bone defect repair induced by malignant osteosarcoma.The resultant composite scaffolds integrated multiple functions involving anti-tumor,osteogenic,and antibacterial potentials,mainly attributed to the anti-tumor effects of SeO_(3)^(2-),osteogenic ef-fects of Sr^(2+)and Zn^(2+),and antibacterial effects of SeO_(3)^(2-)and Zn^(2+).In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells,differentiation of MSCs,and proliferation of MC3T3-E1 while showing excellent antibacterial properties.In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection,and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation.Summarily,the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment,bone defect repair,and post-operative anti-infection,which provided a great potential to be a facile therapeutic material for osteosarcoma resection.

关 键 词：Element-doped HA-PCL scaffolds 3D printing OSTEOGENIC OSTEOSARCOMA ANTIBACTERIAL 

分 类 号：R318.08[医药卫生—生物医学工程]