Bilayer vascular grafts with on-demand NO and H_(2)S release capabilities  

作　　者：Pengfei Li Fubang Liang Lijuan Wang Dawei Jin Yushuang Shang Xu Liu Yanjun Pan Jiang Yuan Jian Shen Meng Yin 

机构地区：[1]Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,Jiangsu Key Laboratory of Bio-functional Materials,Department of Materials Science and Engineering,School of Chemistry and Materials Science,Nanjing Normal University,Nanjing,210023,PR China [2]Department of Cardiothoracic Surgery,Shanghai Children’s Medical Center,School of Medicine,Shanghai Jiao Tong University,1678 Dong Fang Road,Shanghai,200127,PR China [3]Jiangsu Engineering Research Center of Interfacial Chemistry,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,210023,PR China

出　　处：《Bioactive Materials》2024年第1期38-52,共15页生物活性材料（英文）

基　　金：supported by the National Natural Science Fund of China(81873923);Jiangsu Higher Education Institutions(19KJA310001 and PAPD);Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.

摘　　要：Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.

关 键 词：Small-diameter vascular grafts Nitric oxide Hydrogen sulfide KERATIN ENDOTHELIALIZATION 

分 类 号：O64[理学—物理化学]