离子交换柱层析纯化右旋糖酐40提高产品品质  

Purification of Dextran 40 by Ion Exchange Column Chromatography to Improve Product Quality

作　　者：问清江[1] 郑巧霞 孙晓宇[1] 丁浩[1] 慕娟[1] WEN Qing-jiang;ZHENG Qiao-xia;SUN Xiao-yu;DING Hao;MU Juan(Shaanxi Institute of Microbiology,Xi'an 710043,China)

机构地区：[1]陕西省微生物研究所,西安710043

出　　处：《离子交换与吸附》2024年第5期421-428,I0004,共9页Ion Exchange and Adsorption

基　　金：陕西省科学院科学研究专项(项目号2020K-06);西安市科技创新人才服务企业项目(项目号2020KJRC0149)。

摘　　要：右旋糖酐发酵液酶解制备的右旋糖酐40经过离子交换柱层析分离纯化后,相关产品指标达到国际最高标准。右旋糖酐发酵液酶解制备的右旋糖酐40,产率可达31.70 g/L,产品的重均分子量(M_(w))为36125、10%大分子M_(w)为106805、10%小分子M_(w)为7272,达到欧洲标准;阳-阴-阳-阴不连续离子交换柱层析右旋糖酐40产品的炽灼残渣、重金属和氮含量分别为0.01%、0.000643%和0.004%,炽灼残渣和氮含量达到国际最高标准,重金属含量达国家标准,电导率降至70μS/cm以下;阳-阴-阳-阴-阳-阴不连续离子交换柱层析右旋糖酐40的炽灼残渣、重金属和氮含量分别为0.01%、0.0001%和0.005%,均达到国际最高标准,电导率降至10μs/cm以下;3次阳-阴连续离子交换柱层析右旋糖酐40的炽灼残渣、重金属和氮含量分别为0.01%、0.0001%和0.004%,均达到国际最高标准,重均分子量和分子量分布达到欧洲标准,收率超过85%,电导率降至5μS/cm以下。Dextran 40 prepared by enzymatic hydrolysis of dextran fermentation broth was separated and purified by ion exchange column chromatography,achieving the highest international standards for product indexes.Dextran 40 was prepared by enzymatic hydrolysis of dextran fermentation broth,with a yield of 31.70 g/L of fermentation broth.The weight average molecular weight and molecular weight distribution of product were M_(w)36125,10%macromolecular M_(w)106805,and 10%small molecular M_(w)7272,meeting European standards.The contents of ignition residue,heavy metals,and nitrogen in the dextran 40 product obtained by cation–anion-cation-anion discontinuous ion exchange column chromatography were 0.01%,0.000643%,and 0.004%,respectively.The contents of ignition residue and nitrogen met the highest international standards,while the heavy metal content met the national standard.The conductivity dropped below 70μS/cm.The contents of ignition residue,heavy metals,and nitrogen in the Dextran 40 product by cation-anion-cation-anion-cation-anion discontinuous ion exchange column chromatography were 0.01%,0.0001%,and 0.005%,respectively,meeting the highest international standards.The conductivity dropped below 10μS/cm.The ignition residue,heavy metals,and nitrogen contents of dextran 40 obtained from three consecutive cation–anion ion exchange column chromatographies were 0.01%,0.0001%,and 0.004%,respectively,meeting the highest international standards.The weight average molecular weight and molecular weight distribution met European standards.The yield exceeded 85%.The conductivity dropped below 5μS/cm.

关 键 词：离子交换 柱层析 右旋糖酐40 发酵液 酶解 电导率 

分 类 号：TQ464.1[化学工程—制药化工]