Biguanide chitosan microneedles with cell-free DNA scavenging ability for psoriasis therapy  

作　　者：Zihao Liu Yu Wang Yuhan Zhang Liufu Hu Bozhi Chen Yang Li Xindong Guo Bingran Yu Fu-Jian Xu 

机构地区：[1]State Key Laboratory of Chemical Resource Engineering,Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology,Ministry of Education)and Laboratory of Biomedical Materials,Beijing University of Chemical Technology,Beijing 100029,China

出　　处：《Bioactive Materials》2024年第3期497-505,共9页生物活性材料（英文）

基　　金：supported by National Key R&D Program of China(Grant number 2021YFB3800900);National Natural Science Foundation of China(Grant numbers 52073013,22122501,52221006 and 52161145410);Beijing Outstanding Young Scientist Program(No.BJJWZYJH01201910010024);approved by the Ethical Committee of Chinese Academy of Medical Sciences(CAMS).

摘　　要：High levels of cell-free DNA(cfDNA)induce psoriasis.Currently,the treatment of psoriasis has the disadvantages of penetration difficulty,suppression of normal immunity,and skin irritation.In this study,biguanide chitosan microneedles(BGC-MNs)were prepared to treat psoriasis by removing cfDNA from the dermis through the skin barrier.The effects of chitosan with different bisguanidine contents on DNA-binding capacity,biocompatibility,and inflammation inhibition were compared,revealing that chitosan containing 20%bisguanidine(BGC2)was found to have the best overall performance.In vitro,BGC2 effectively cleared cfDNA and inhibited the production of inflammatory factors.BGC-MN made from BGC2 had good mechanical and solubility properties.In vivo,BGC-MNs cleared cfDNA,reduced the level of inflammatory factors in the dermis,and exerted a good therapeutic effect on mice with psoriasis.These results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.

关 键 词：Biguanide chitosan cfDNA MICRONEEDLES PSORIASIS 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]