Nano oxygen chamber by cascade reaction for hypoxia mitigation and reactive oxygen species scavenging in wound healing  

作　　者：Xiaoxue Han Leah Ju Chai Saengow Wen Ren Randy Ewoldt Timothy Fan Joseph Irudayaraj 

机构地区：[1]Department of Bioengineering,1102 Everitt Lab,1406 W.Green St.,University of Illinois at Urbana-Champaign,Urban1,IL,61801,USA [2]Biomedical Research Center,Mills Breast Cancer Institute,Carle Foundation Hospital,Urban1,IL,61801,USA [3]Cancer Center at Illinois,Beckman Institute,Urban1,IL,61801,USA [4]Carle Illinois College of Medicine,University of Illinois at Urbana-Champaign,Urban1,IL,61801,USA [5]Department of Veterinary Clinical Medicine,College of Veterinary Medicine,University of Illinois at Urbana-Champaign,Urban1,IL,61801,USA [6]Department of Mechanical Science and Engineering,University of Illinois at Urbana-Champaign,Urban1,IL,USA

出　　处：《Bioactive Materials》2024年第5期67-81,共15页生物活性材料（英文）

摘　　要：Hypoxia,excessive reactive oxygen species(ROS),and impaired angiogenesis are prominent obstacles to wound healing following trauma and surgical procedures,often leading to the development of keloids and hypertrophic scars.To address these challenges,a novel approach has been proposed,involving the development of a cascade enzymatic reaction-based nanocarriers-laden wound dressing.This advanced technology incorporates superoxide dismutase modified oxygen nanobubbles and catalase modified oxygen nanobubbles within an alginate hydrogel matrix.The oxygen nano chamber functions through a cascade reaction between superoxide dismutase and catalase,wherein excessive superoxide in the wound environment is enzymatically decomposed into hydrogen peroxide,and this hydrogen peroxide is subsequently converted into oxygen by catalase.This enzymatic cascade effectively controls wound inflammation and hypoxia,mitigating the risk of keloid formation.Concurrently,the oxygen nanobubbles release oxygen continuously,thus providing a sustained supply of oxygen to the wound site.The oxygen release from this dynamic system stimulates fibroblast proliferation,fosters the formation of new blood vessels,and contributes to the overall wound healing process.In the rat full-thickness wound model,the cascade reaction-based nano oxygen chamber displayed a notable capacity to expedite wound healing without scarring.Furthermore,in the pilot study of porcine full-thickness wound healing,a notable acceleration of tissue repair was observed in the conceived cascade reaction-based gel treated group within the 3 days post-surgery,which represents the proliferation stage of healing process.These achievements hold significant importance in ensuring the complete functional recovery of tissues,thereby highlighting its potential as a promising approach for enhancing wound healing outcomes.

关 键 词：HYPOXIA ROS scavenging ANTI-INFLAMMATION Oxygen nanobubble Scarless wound healing 

分 类 号：O64[理学—物理化学]