番茄红素对大气细颗粒物暴露所致大鼠肺氧化损伤的保护作用  

The protective effect of lycopene on lung oxidative damage induced by atmospheric fine particulate matter exposure in rats

作　　者：韩涛 杨艳晖[2] 潘洪志[3] Han Tao;Yang Yanhui;Pan Hongzhi(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Second Medical College,Harbin Medical University,Harbin 150086,China;Shanghai University of Medicine&Health Sciences,Shanghai 200237,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]哈尔滨医科大学附属第二医院,哈尔滨150086 [3]上海健康医学院,上海200237

出　　处：《卫生研究》2024年第6期999-1006,共8页Journal of Hygiene Research

基　　金：中国营养学会营养科研基金-帝斯曼专项科研基金(No.CNS-DSM2018A33)。

摘　　要：目的 探讨番茄红素对大气细颗粒物(fine particulate matter, PM_(2.5))暴露所致大鼠肺氧化损伤的保护作用。方法 选用纯种7周龄雄性SD大鼠60只,随机分为6组:正常对照组,PM_(2.5)染毒对照组,维生素E干预组,番茄红素低、中、高剂量干预组(5、15、30 mg/kg),每组10只。除正常对照组外,其余各组大鼠气管滴注PM_(2.5)悬浮液染毒(7.5 mg/kg),正常对照组滴注等量生理盐水。染毒每周3次,每次间隔24小时,从染毒第1日起,番茄红素干预组每日灌胃溶有番茄红素的色拉油,维生素E干预组每日灌胃溶有维生素E(7.5 mg/kg)的色拉油,其他两组灌胃相同体积的色拉油,连续4周后脱颈处死全部大鼠,制作肺病理切片并收集血清和肺组织,肺组织制备成组织匀浆,离心后收集上清液。采用分光光度法分别测定大鼠血清及肺组织中谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽(glutataione, GSH)、总抗氧化能力(total antioxidant capacity, T-AOC)、丙二醛(malondialdehyde, MDA)、谷胱甘肽转硫酶(glutathione-S-transferase, GST)、过氧化氢酶(catalase, CAT)、羟自由基(hydroxyl radical, OH·)、一氧化氮(nitric oxide, NO)的含量;采用酶联免疫吸附试验法测定血清和肺组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和8-羟基脱氧鸟苷(8-hydroxy-2 deoxyguanosine, 8-OHdG)含量,并观察大鼠肺组织病理改变。结果 与正常对照组相比,PM_(2.5)染毒组血清GSH[(9.47±1.37)mg/g vs.(11.10±3.82)mg/g]、SOD[(9.43±2.41)U/mg vs.(13.82±4.64)U/mg]、CAT[(5.35±1.88)U/mg vs.(9.54±3.06)U/mg]、8-OHdG[(5.52±1.66)μg/g vs.(4.76±1.01)μg/g]含量及肺组织匀浆GSH[(2.19±0.29)mg/g vs.(3.18±0.49)mg/g]、SOD[(23.98±4.20)U/mg vs.(31.95±5.08)U/mg]、CAT[(5.85±1.95)U/mg vs.(3.57±1.96)U/mg]、GSH-Px[(49.15±7.01)mg/g vs.(68.46±11.15)mg/g]、OH·[(74.02±12.84)U/mg vs.(51.03±14.85)UOBJECTIVE To investigate the protective effect of lycopene on lung oxidative damage induced by atmospheric fine particulate matter(PM_(2.5))in rats.METHODS Sixty 7-week-old male Sprague-Dawley rats were randomly divided into six groups:normal control group,PM_(2.5) model group,reference control group(vitamin E dose group,7.5 mg/kg),and three lycopene groups with low-dose(5 mg/kg),medium dose(15 mg/kg)and high dose(30 mg/kg),with 10 rats in each group.Except for the control group,all groups were exposed to PM_(2.5) suspension intratracheally(equivalent to 7.5 mg/kg),poisoning 3 times a week,with an interval of 24 hours between each test.The vitamin E intervention group dissolved the salad oil with vitamin E by daily gavage,while the control group received an equal amounts of physiological saline.From the first day of exposure,lycopene was given daily via gavage for 4 weeks.Lycopene groups received lycopene dissolved in salad oil,while control and model groups received salad oil of the same volume.After 4 weeks,all rats were killed.Lung pathology sections were made and serum and lung tissue were collected and homogenized,centrifuge and collect the supernatant.Using UV visible spectrophotometry to measure levels of glutathione peroxidase(GSH-Px),glutataione(GSH),superoxide dismutase(SOD),total antioxidant capacity(T-AOC),malondialdehyde(MDA),glutathione S-transferase(GST),catalase(CAT),hydroxyl radical(OH),nitric oxide(NO).Using enzymelinked immunosorbent Assays(ELISA)to measure levels of tumor necrosis facto-α(TNFα),interleukin-6(IL-6),and 8-hydroxy-2-deoxyguanosine(8-OHdG).Lung histopathology was also observed.RESULTS Compared to the normal control group,there were significant differences in the content of GSH((9.47±1.37)mg/g vs.(11.10±3.82)mg/g),SOD((9.43±2.41)U/mg vs.(13.82±4.64)U/mg),CAT((5.35±1.88)U/mg us.(9.54±3.06)U/mg),8-0HdC((5.52±1.66)μg/g vs.(4.76±1.01)μg/g)in the serum,and GSH(2.19±0.29)mg/g us.(3.18±0.49)mg/g),SOD((23.98±4.20)U/mg us.(31.95±5.08)U/mg),CAT((5.85±1.95)U/mg vs.(3.57±1.96

关 键 词：细颗粒物 番茄红素 肺氧化损伤 氧化应激 

分 类 号：R151[医药卫生—营养与食品卫生学]