基于微阵列分析DDX5影响头颈部鳞状细胞癌的初步探究  

Preliminary investigation of microarray-based analysis of DDX5 affecting head and neck squamous cell carcinoma

作　　者：刘郭琦 刘春霞[1] 王静静[1] 左金华[1] 王芳[1] 宋娇娇 于栋林 马向瑞 王文龙 LIU Guoqi;LIU Chunxia;WANG Jingjing;ZUO Jinhua;WANG Fang;SONG Jiaojiao;YU Donglin;MA Xiangrui;WANG Wenlong(Department of Oral and Maxillofacial Surgery,Affiliated Hospital to Binzhou Medical University,256699,China)

机构地区：[1]滨州医学院附属医院口腔颌面外科,256699

出　　处：《实用口腔医学杂志》2024年第6期810-816,共7页Journal of Practical Stomatology

基　　金：山东省自然科学基金(编号:ZR2018BH026,ZR2019PH07-5);山东省中医药科技青年项目(编号:2020Q062);山东省医药卫生科技发展计划项目(编号:202008020682)。

摘　　要：目的:探讨DDX5解旋酶(DDX5)在头颈部鳞状细胞癌(HNSCC)中的表达及作用。方法:组织微阵列芯片分析DDX5相关mRNA表达谱数据,并用R软件筛选差异mRNAs(DEGs)。使用GEPIA 2、TCGA数据库预测DDX5的表达水平。通过RT-qPCR、免疫组化、Western blot检测DDX5表达水平。基于高通量测序数据筛选差异circRNAs(DECs)、差异miRNAs(DEMIs)构建DDX5竞争内源性RNA网络(ceRNA)。Cytoscape软件可视化ceRNA网络图,进一步筛选出调控轴。结果:组织芯片筛选发现HNSCC中DDX5表达上调。免疫组化验证DDX5阳性染色更集中于鳞癌细胞核;qPCR结果提示DDX5 mRNA在组织和细胞中的表达有临床意义(P<0.05);Western blot结果示DDX5蛋白在组织中高表达。在ceRNA网络中确定了相关HNSCC轴circRNA-039626-miR-222-5p-DDX5。结论:DDX5在HNSCC中高表达,并且circRNA-039626-miR-222-5p-DDX5轴可能是HNSCC发生发展的潜在调控轴。Objective:To investigate the expression and role of DEAD-box RNA helicases 5(DDX5 helicases)in head and neck squamous carcinoma(HNSCC).Methods:Tissue microarray microarray was used to assess relevant mRNA expression profile data,and R software was used to screen differential mRNAs(DEGs).The expression level of DDX5 was predicted using GEPIA 2,TCGA databases,and detected by immunohistochemistry,western blot and RT-qPCR in the HNSCC tissue and cell lines.Based on high-throughput sequencing data of DECs,differentially expressed miRNAs(DEMIs)relevant DDX5 competitive endogenous RNA network(ceRNA)was constructed.The software cytoscape was used to visualize the ceRNA network map and further screen the regulatory axis.Results:The results of microarray screening revealed that DDX5 expression in HNSCC was upregulated.Immunohistochemistry verified that DDX5 was stronger expressed in the nuclei of squamous carcinoma cells.qPCR results suggested that significant expression of DDX5 mRNA at the tissue and cellular levels(P<0.05).Western blot results showed high expression of DDX5 protein in the tissues.The ceRNA network was constructed,from which the relevant HNSCC axis circRNA-039626-miR-222-5p-DDX5 was identified.Conclusion:DDX5 is highly expressed in HNSCC,and the circRNA-039626-miR-222-5p-DDX5 axis may be a potential regulatory axis for the development of HNSCC.

关 键 词：DDX5解旋酶 头颈部鳞状细胞癌 ceRNA 微阵列分析 

分 类 号：R739.8[医药卫生—肿瘤]