The crucial role of fish mucus in regulating progeny inflammation and microbial homeostasis  

作　　者：Zi-Xuan Wang Hao-Yue Xu Qiu-Lu He Yong-Yao Yu Zhen Xu 

机构地区：[1]Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,China [3]College of Animal Science and Technology,Yangtze University,Jingzhou,434024,China [4]Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China

出　　处：《Water Biology and Security》2024年第2期31-45,共15页水生生物与安全（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32225050,32073001)to Z.X.

摘　　要：Maternal immunity plays a vital role in protecting offspring from pathogen invasion,and it is present in nearly all vertebrates.As the most ancient vertebrates,teleost fish continuously secrete a substantial amount of mucus due to their unique living environment,and often exhibit schooling behavior.Previous studies have highlighted the presence of numerous immune components in the mucus of fish,that plays a crucial role in resisting pathogens.We hypothesize that this represents a distinctive maternal immune response mechanism in fish.In this study,we established a dextran sulfate sodium(DSS)-induced injury model by immersing zebrafish larvae in 0.5%DSS.This elicited robust inflammatory responses and showed DSS-induced alterations in microbial abundance via 16S rRNA sequencing.These changes included the disruption of normal gut structure;an increase in goblet cells;increased expression of inflammatory cytokines;and infiltration of neutrophils and macrophages into the gut,as well as an increase in pathogenic bacteria and a decline in probiotic bacteria.Furthermore,we conducted a mucus protection test by adding adult zebrafish mucus in fish tank water containing 0.5%DSS to test our hypothesis.As suspected,the mucus from adult zebrafish exhibited a protective role in defending zebrafish larvae against DSSinduced enteritis by alleviating excessive inflammatory responses and increasing probiotic abundance.In conclusion,our results confirm a significant role of adult fish mucus in the immune response of teleost fish to DSSinduced inflammation.Collectively,our findings show that the mucus of adult zebrafish may represent a novel form of maternal immunization,playing roles analogous to mammalian milk in immune regulatory functions.

关 键 词：MUCUS Maternal immunity Inflammatory response Dextran sulfate sodium(DSS) ZEBRAFISH 

分 类 号：Q95[生物学—动物学]