香菇多糖通过IL-6/STAT3通路对AOM/DSS诱导结肠炎相关结直肠癌的抑制作用及机制  

Inhibitory Effect and Mechanism of Lentinan on Colitis-Associated Colorectal Cancer Induced by AOM/DSS Through IL-6/STAT3 Pathway

作　　者：刘俊杰 梁家 庞天舒 薛佳龙 刘德纯[1] LIU Junjie;LIANG Jia;PANG Tianshu;XUE Jialong;LIU Dechun(Department of Gastrointestinal Surgery,The First Affiliated Hospital(College of Clinical Medicine)of Henan University of Science and Technology,Luoyang 471000,China)

机构地区：[1]河南科技大学临床医学院(第一附属医院)胃肠外科,洛阳471000

出　　处：《肿瘤防治研究》2024年第11期908-912,共5页Cancer Research on Prevention and Treatment

摘　　要：目的 研究香菇多糖通过白细胞介素6/信号转导及转录激活蛋白3(IL-6/STAT3)通路对偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)诱导结肠炎相关结直肠癌(CAC)的抑制作用及机制。方法 C57BL/6小鼠随机分为对照组、模型组、低剂量组(0.865 mg/kg香菇多糖)、中剂量组(1.73 mg/kg香菇多糖)、高剂量组(3.46 mg/kg香菇多糖)。除对照组外,其余各组采用AOM/DSS诱导CAC,造模过程中给予相应药物腹腔注射干预。比较各组小鼠体质量、疾病活动度(DAI)评分、结肠长度、肿瘤数目,苏木精伊红染色观察结肠组织病理形态,ELISA法检测血清中IL-6及IL-1β、IL-18含量,Western blot检测结肠组织中IL-6、p-STAT3、c-Myc表达。结果 模型组肿瘤数目、DAI评分、血清IL-6、IL-1β、IL-18含量及结肠组织中IL-6、p-STAT3、c-Myc的表达水平均高于对照组,体质量、结肠长度低于对照组(P<0.05),病理形态显示结肠组织可见腺癌形成;不同剂量香菇多糖干预后,肿瘤数目、DAI评分、血清IL-6、IL-1β、IL-18含量及结肠组织中IL-6、 p-STAT3、 c-Myc的表达水平均低于模型组,体质量、结肠长度高于模型组(P<0.05),病理形态显示结肠组织可见不同级别腺瘤,未出现腺癌。结论 香菇多糖抑制CAC的形成,其抑癌作用与抑制IL-6/STAT3通路有关。Objective To investigate the inhibitory effect and mechanism of lentinan on colitis-associated colorectal cancer(CAC)induced by azomethane(AOM)/dextran sulfate sodium salt(DSS)through the IL-6/STAT3 pathway.Methods C57BL/6 mice were randomly divided into a control group,a model group,a low-dose group(0.865 mg/kg lentinan),a medium-dose group(1.73 mg/kg lentinan),and a high-dose group(3.46 mg/kg lentinan).Except the control group,CAC was induced by AOM/DSS in the other groups,and corresponding drugs were injected intraperitoneally during the modeling process.Body mass,disease activity index(DAI)score,colon length,and tumor number were compared among all groups.Hematoxylin–eosin staining was used to observe the pathological morphology of colon.ELISA was utilized to detect the IL-6,IL-1β,and IL-18 contents in serum.Western blot analysis was conducted to detect the expression levels of IL-6,p-STAT3,and c-Myc in colon tissues.Results The tumor number,DAI score,serum IL-6,IL-1β,and IL-18 contents and the expression levels of IL-6,p-STAT3,and c-Myc in the colon tissue of the model group were higher than those of the control group,while the body mass and colon length were lower than those of the control group(P<0.05).The pathological morphology of colon tissues showed adenocarcinoma formation.After different doses of lentinan intervention,the tumor number,DAI score,serum IL-6,IL-1β,and IL-18 contents and the expression levels of IL-6,p-STAT3,and c-Myc in colon tissues were all lower than those in the model group,while body mass and colon length were higher than those in the model group(P<0.05).The pathological morphology of colon tissues showed adenomas of different grades but no adenocarcinoma was found.Conclusion Lentinan inhibits CAC formation,and its anticancer effect is related to the inhibition of the IL-6/STAT3 pathway.

关 键 词：香菇多糖 结肠炎相关结直肠癌 白细胞介素-6 信号转导及转录激活蛋白3 

分 类 号：R735.35[医药卫生—肿瘤]