Accelerated infected wound healing by probiotic-based living microneedles with long-acting antibacterial effect  

作　　者：Yinli Jin Yun Lu Xue Jiang Min Wang Yaqi Yuan Yongnian Zeng Liang Guo Wei Li 

机构地区：[1]Department of Thyroid and Breast Surgery,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,430071,China [2]TaiKang Center for Life and Medical Sciences,Wuhan University,Wuhan,China [3]Department of Plastic Surgery,Zhongnan Hospital of Wuhan University,Wuhan,China [4]Department of Pharmacy,Zhongnan Hospital of Wuhan University,Wuhan,China

出　　处：《Bioactive Materials》2024年第8期292-304,共13页生物活性材料（英文）

基　　金：support from the National Natural Science Foundation of China(NSFC,No.82373798,No.52103182);Youth Interdisciplinary Special Fund of Zhongnan Hospital of Wuhan University(Grant No.ZNQNJC2023002);Young Talents Project of Hubei Province Administration of Traditional Chinese Medicine(No.ZY2023Q014).

摘　　要：Delays in infected wound healing are usually a result of bacterial infection and local inflammation,which imposes a significant and often underappreciated burden on patients and society.Current therapies for chronic wound infection generally suffer from limited drug permeability and frequent drug administration,owing to the existence of a wound biofilm that acts as a barrier restricting the entry of various antibacterial drugs.Here,we report the design of a biocompatible probiotic-based microneedle(MN)patch that can rapidly deliver beneficial bacteria to wound tissues with improved delivery efficiency.The probiotic is capable of continuously producing antimicrobial substances by metabolizing introduced glycerol,thereby facilitating infected wound healing through long-acting antibacterial and anti-inflammatory effects.Additionally,the beneficial bacteria can remain highly viable(>80%)inside MNs for as long as 60 days at 4℃.In a mouse model of Staphylococcus aureus-infected wounds,a single administration of the MN patch exhibited superior antimicrobial efficiency and wound healing performance in comparison with the control groups,indicating great potential for accelerating infected wound closure.Further development of live probiotic-based MN patches may enable patients to better manage chronically infected wounds.

关 键 词：MICRONEEDLES Infected wound healing Probiotic Transdermal delivery LONG-ACTING 

分 类 号：O17[理学—数学]