Targeting SRSF10 might inhibit M2 macrophage polarization and potentiate anti-PD-1 therapy in hepatocellular carcinoma  

作　　者：Jialiang Cai Lina Song Feng Zhang Suiyi WuGuiqi Zhu Peiling Zhang Shiping Chen Junxian Du Biao Wang Yufan Cai Yi Yang Jinglei Wan Jian Zhou Jia Fan Zhi Dai 

机构地区：[1]Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai,P.R.China [2]State Key Laboratory of Genetic Engineering,Fudan University,Shanghai,P.R.China [3]Key Laboratory of Carcinogenesis and Cancer Invasion,Fudan University,Ministry of Education,Shanghai,P.R.China [4]Department of Gastroenterology and Hepatology,Zhongshan Hospital,Fudan University,180 Fenglin Road,Shanghai,P.R.China [5]Shanghai Institute of Liver Disease,Shanghai,P.R.China [6]Department of Liver Surgery and Transplantation,Zhongshan Hospital,Fudan University,Shanghai,P.R.China [7]Research Unit of Liver Cancer Recurrence and Metastasis,Chinese Academy of Medical Sciences,Beijing,P.R.China [8]Department of general surgery,Zhongshan Hospital,Fudan University,Shanghai,P.R.China [9]Department of Radiation Oncology,Zhongshan Hospital,Fudan University,Shanghai,P.R.China

出　　处：《Cancer Communications》2024年第11期1231-1260,共30页癌症通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(No.82372946 and No.82072670);the 12 Leading Project of the Science and Technology Committee of Shanghai Municipality(No.21Y21900100);the Project of Shanghai Municipal Health Commission(No.202140269).

摘　　要：Background:The efficacy of immune checkpoint blockade therapy in patients with hepatocellular carcinoma(HCC)remains poor.Although serine-and arginine-rich splicing factor(SRSF)family members play crucial roles in tumors,their impact on tumor immunology remains unclear.This study aimed to elucidate the role of SRSF10 in HCC immunotherapy.Methods:To identify the key genes associated with immunotherapy resistance,we conducted single-nuclear RNA sequencing,multiplex immunofluorescence,and The Cancer Genome Atlas and Gene Expression Omnibus database analyses.We investigated the biological functions of SRSF10 in immune evasion using in vitro co-culture systems,flow cytometry,various tumor-bearing mouse models,and patient-derived organotypic tumor spheroids.Results:SRSF10 was upregulated in various tumors and associated with poor prognosis.Moreover,SRSF10 positively regulated lactate production,and SRSF10/glycolysis/histone H3 lysine 18 lactylation(H3K18la)formed a positive feedback loop in tumor cells.Increased lactate levels promoted M2 macrophage polarization,thereby inhibiting CD8^(+)T cell activity.Mechanistically,SRSF10 interacted with the 3′-untranslated region of MYB,enhancing MYB RNA stability,and subsequently upregulating key glycolysis-related enzymes including glucose transporter 1(GLUT1),hexokinase 1(HK1),lactate dehydrogenase A(LDHA),resulting in elevated intracellular and extracellular lactate levels.Lactate accumulation induced histone lactylation,which further upregulated SRSF10 expression.Additionally,lactate produced by tumors induced lactylation of the histone H3K18la site upon transport into macrophages,thereby activating transcription and enhancing pro-tumor macrophage activity.M2 macrophages,in turn,inhibited the enrichment of CD8^(+)T cells and the proportion of interferon-γ+CD8^(+)T cells in the tumor microenvironment(TME),thus creating an immunosuppressive TME.Clinically,SRSF10 could serve as a biomarker for assessing immunotherapy resistance in various solid tumors.Pharmacological targeting

关 键 词：GLYCOLYSIS Histone lactylation Immune checkpoint blockade Serine and arginine rich splicing factor 10 Tumor-Associated Macrophage 

分 类 号：R735.7[医药卫生—肿瘤]