The effect of feed additive ethoxyquin on tilapia growth performance and potential secondary hazard to consumers  

作　　者：Shanjun Chen Chong Wang Yanan Liu Zhigang Zhou Linglin Fu Yanbo Wang 

机构地区：[1]Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China [2]Sino-Norway Joint Lab on Fish Gut Microbiota,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]School of Food and Health,Beijing Technology and Business University(BTBU),Beijing 100048,China

出　　处：《Food Science and Human Wellness》2024年第6期3236-3244,共9页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32061133004);the Major Project of Digital Plus Discipline Construction of Zhejiang Gongshang University(SZJ2022A010);the Zhejiang Provincial Natural Science Foundation of China(LGN21C200013).

摘　　要：Ethoxyquin(EQ)is a widely used feed additives for aquaculture,but the potential hazard to fish and consumers are not fully understood.In this work,EQ was applied to tilapia fed with either high-fat or low-fat feed,and the changes in growth performance and intestinal barrier integrality of genetically improved farmed tilapia was observed.Additionally,the microbiota in the intestinal content and flesh was analyzed by 16S rDNA sequencing,and the flesh metabolites was measured by LC-MS/MS untargeted metabolomics.In the low-fat group,EQ increased weight gain and feed conversion rate,but reduced survival rate.In the high-fat group,only weight gain decreased,and there was no significant effect on feed conversion rate and survival rate.Additionally,the intestinal villi and microbiota diversity was impaired in both feeding conditions.Microbiota analysis revealed that EQ resulted in different composition and lower diversity of the flesh microbiota and upregulated the proportion of potential pathogens.EQ also changed the flesh metabolome,decreasing beneficial metabolites and increasing potential harmful components.This work elucidated the effect of EQ on growth performance of tilapia and implied that it has adverse effects on tilapia and may be passed on to consumers.

关 键 词：Ethoxyquin TILAPIA Feed MICROBIOTA METABOLOME 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程] S965.125[理学—分析化学]