Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice  

作　　者：Muni Swamy Ganjayi Karunakaran Reddy Sankaran Balaji Meriga Ruchika Bhatia Shikha Sharma Kanthi Kiran Kondepudi 

机构地区：[1]Department of Biochemistry,Sri Venkateswara University,Tirupati 517502,India [2]Department of Biomedical Sciences,Heritage College of Osteopathic Medicine,Ohio University,Athens 45701,United States [3]Food and Nutrition Biotechnology Division,National Agri-Food Biotechnology Institute,SAS Nagar,Punjab 140306,India

出　　处：《Food Science and Human Wellness》2024年第6期3256-3265,共10页食品科学与人类健康（英文）

基　　金：Authors are grateful to the Department of Science and Technology,New Delhi,for financial support(DST-SERB,EEQ/2016/000123,2016-17),and thank DST-FIST,Department of Biochemistry,S.V.University.

摘　　要：In the present study we investigated the impact of the combination of astragalin and rutin(CAR)on restoring gut-microbial dysbiosis and obesity and obesity related disorders.Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet(HFD)for 16 weeks.Compared to vehicle treated group(HFD group),CAR could substantially improve selected gut microbiota abundance(Akkermansia,Lactobacillus,Bifidobacteria,Roseburia,Prevotella),reversed the Firmicutes/Bacteroidetes proportions,and inhibited the growth of Escherichia coli,Salmonella,and Klebsiella in obese mice.In addition,CAR-treated mice showed significantly increased total short-chain fatty acid production,reduced body weight gain,organs’weights,serum lipid profile(except HDL)and insulin resistance.The mRNA expressions of CCAAT/enhancer binding protein-α(C/EBP-α),sterol regulatory element-binding protein-1c(SREBP-1c),peroxisome proliferator-activated receptor-γ(PPAR-γ),acetyl-CoA carboxylase(ACC),adipocyte protein 2(aP2),and fatty acid synthase(FAS)were downregulated(P<0.05)and the protein expression of PPAR-γwas downregulated while adenosine 5’monophosphate-activated protein kinase(AMPK)was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group.Interestingly,CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues.In conclusion,the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders.

关 键 词：ASTRAGALIN Gut microbiota Moringa oleifera OBESITY RUTIN 

分 类 号：TS201.4[轻工技术与工程—食品科学] R589.2[轻工技术与工程—食品科学与工程]