Soy polysaccharide maintains colonic homeostasis to protect from dextran sulphate sodium-induced colitis by modulating gut microbiota and intestinal epithelial regeneration  

作　　者：Nana Zhang Jianlin Liu Weiyue Zhang Xinxin Guo Shuying Li Hongtai Zhang Minjie Wang Bei Fan Fengzhong Wang 

机构地区：[1]Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Key Laboratory of Agro-Products Processing,Ministry of Agriculture,Beijing 100095,China [2]School of Basic Medical Sciences,Inner Mongolia Medical University,Hohhot 010107,China

出　　处：《Food Science and Human Wellness》2024年第6期3284-3300,共17页食品科学与人类健康（英文）

基　　金：funded by National Natural Science Foundation of China(NSFC32372350);the Knowledge Innovation Program Funding of Institute of Food Science and Technology(CAASASTIP2021-IFST);China Agriculture Research System(CARS-04);Agricultural Science and Technology Innovation Program of Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences(CAAS-ASTIP-G2022-IFST-06).

摘　　要：Soy polysaccharide(SP)has been reported to possess the properties of modulating gut microbiome diversity.Here,we aimed to explore the protective effects of SP against dextran sulphate sodium(DSS)-induced colitis.Pre-treatment with SP at a dosage of 400 mg/kg·day alleviated colitis symptoms,preventing the weight loss and colon shorten.SP suppressed DSS-induced inflammatory response and enhanced M1 to M2 macrophage polarization.Further investigation showed that SP significantly promoted the regeneration of crypt and the expansion of goblet cell production.In addition,bacterial 16S rRNA sequencing analysis showed that SP modulated the composition of fecal microbiota,including selectively increasing Lactobacillus relative abundance.Notably,SP treatment enriched the production of Lactobacillus-derived lactic acid,which was sensed by its specific G-protein-coupled receptor 81(Gpr81)/Wnt3/β-catenin signaling,and promoted the regeneration of intestinal stem cells.Fecal microbiome transplantation demonstrated that intestinal flora partially contributed to the beneficial effects of SP on preventing against colitis.In conclusion,SP exhibited the protective effects against colitis,which could be partly associated with modulating the composition of gut microbiota and enrichment of lactic acid.This study suggests that SP has potential to be developed as nutritional intervention to prevent colitis.

关 键 词：Soy polysaccharide COLITIS Gut microbiota Lactic acid Proliferation Intestinal stem cells 

分 类 号：R574.62[医药卫生—消化系统] TS201.4[医药卫生—内科学]