Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy  

作　　者：Hongyu Pan Qianwang Zheng Yuan Zou Guozhao Luo Mingzhen Tu Na Wang Jingru Zhong Liqiong Guo Junfang Lin 

机构地区：[1]College of Food Science,South China Agricultural University,Guangzhou 510640,China [2]Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China [3]Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province,Guangzhou 510640,China [4]Guangzhou Alchemy Biotechnology Co.,Ltd.,Guangzhou 510760,China

出　　处：《Food Science and Human Wellness》2024年第6期3434-3446,共13页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32072646,31772373,and 32001832).

摘　　要：Ergothioneine is a natural amino acid with powerful antioxidant activity.Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans,and enhance telomerase activity.In present study,the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies.And the alterations in the gut barrier,gut morphology,and other gut physiological indicators of aging flies were evaluated.Here,the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life,and significant alterations in the gut microbiota populations were only observed in flies at late-life.And ergothioneine attenuated age-related gut injuries,including decreasing reactive oxygen species levels in intestinal enterocytes,reduction of intestinal cells death,and preservation of gut integrity in aging flies.Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy.And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism.

关 键 词：ERGOTHIONEINE Gut microbiota Intestinal autophagy Intestinal barrier Drosophila melanogaster 

分 类 号：TS201.21[轻工技术与工程—食品科学]