The dynamics of PEG-coated nanoparticles in concentrated protein solutions up to the molecular crowding range  

作　　者：Ferdinand Otto Francesco Dallari Fabian Westermeier D.C.Florian Wieland Wolfgang J.Parak Felix Lehmkühler Florian Schulz 

机构地区：[1]Center for Hybrid Nanostructures,Universität Hamburg,Hamburg,Germany [2]Department of Physics and Astronomy“Galileo Galilei”,University of Padova,Padova,Italy [3]Deutsches Elektronen-Synchrotron DESY,Hamburg,Germany [4]Institute of Metallic Biomaterials,Helmholtz-Zentrum Hereon,Geesthacht,Germany [5]The Hamburg Centre for Ultrafast Imaging,Hamburg,Germany

出　　处：《Aggregate》2024年第3期209-218,共10页聚集体（英文）

基　　金：Deutsche Forschungsgemeinschaft,Grant/Award Numbers:GRK 2536,EXC 2056;European Synchrotron Radiation Facility,Grant/Award Number:SC 5173。

摘　　要：Polymer-coated nanoparticles are widely studied in the context of nanomedicine and it is therefore of utmost importance to understand not only how their struc-ture but also how their colloidal dynamics are affected by physiologically relevant conditions.A characteristic feature of the cytosol of cells is the very high concen-tration of proteins among other matrix components,often termed macromolecular crowding.Here,the structure and colloidal dynamics of poly(ethylene glycol)(PEG)-coated gold nanoparticles in the presence of bovine serum albumin(BSA)concentrations ranging from 0 to 265 mg/mL are studied with X-ray photon correla-tion spectroscopy.For protein–nanoparticle mixtures with high BSA concentrations,comparable to intracellular levels,a significant deviation of the apparent viscosity from expectations for pure BSA solutions is found.Thefindings strongly indicate that the nanoscopic viscous properties of the dense protein solutions are significantly affected by the nanoparticles.At these high concentrations,the colloidal stability of the samples depends on the molecular weight of the coating PEG–ligand,whereas at lower concentrations no differences are observed.

关 键 词：AGGREGATION NANOPARTICLES PEG POLYMERS PROTEINS XPCS 

分 类 号：TB383[一般工业技术—材料科学与工程] O313[理学—一般力学与力学基础]