Multifunctional inverse opal microcarriers-based cytokines delivery system with stem cell homing capability for osteoarthritis treatment  

作　　者：Lingyu Sun Jingjing Gan Lijun Cai Feika Bian Wei Xu Yuanjin Zhao 

机构地区：[1]Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing,China [2]Department of Orthopedics,Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China [3]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision,and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,China

出　　处：《Aggregate》2024年第4期199-207,共9页聚集体（英文）

基　　金：National Key Research and Development Program of China,Grant/Award Number:2020YFA0908200;National Natural Science Foundation of China,Grant/Award Numbers:T2225003,52073060,61927805,82302398,82272165;Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2021B1515120054;Shenzhen Fundamental Research Program,Grant/Award Numbers:JCYJ20190813152616459,JCYJ20210324133214038;Jiangsu Funding Program for Excellent Postdoctoral Talent,Grant/Award Number:2022ZB710;China Postdoctoral Science Foundation,Grant/Award Numbers:2022TQ0145,2022M721581。

摘　　要：Osteoarthritis has been regarded as a complex and serious degenerative disease.Attempts in this area are focused on improving the curative effect of stem cell-based therapies.In this work,we present a novel inverse opal microcarriers-based cytokines delivery system to induce autologous stem cell homing for osteoarthritis treatment.Considering their important role in stem cell recruitment and chondro-genic differentiation respectively,platelet-derived growth factor BB(PDGF-BB)and transforming growth factorβ3(TGF-β3)are loaded into inverse opal microcarriers as model cytokines.Since cytokine release induces the corresponding variations in characteristic reflection spectra and structural colors,the inverse opal microcarriers possess the optical self-reporting capacity to monitor the release process.In vitro cell experiments reveal that inverse opal microcarriers could successfully recruit the gathering of mesenchymal stem cells through the release of loaded cytokines.Based on these features,we have demonstrated the enhanced therapeutic effect of PDGF-BB and TGF-β3 loaded inverse opal microcarriers in the treatment of rat osteoarthritis models.These results indicate that the multifunctional inverse opal microcarriers-based cytokines delivery system wouldfind broad prospects in osteoarthritis treatment and other biomedicalfields.

关 键 词：CYTOKINE hydrogel inverse opal MICROFLUIDICS OSTEOARTHRITIS stem cell homing 

分 类 号：R68[医药卫生—骨科学]