QuEChERS-气相色谱-三重四极杆质谱法同时测定保健食品中8种N-亚硝胺  

Simultaneous Determination of Eight N-nitrosamines in Health Foods by QuEChERS-Gas Chromatography-Triple Quadrupole Mass Spectrometry

作　　者：徐华伟 王婷 程莎 XU Huawei;WANG Ting;CHENG Sha(Qingdao Product Quality Testing Research Institute,Qingdao 266000,China;Qingdao Food Inspection Institute,Qingdao 266000,China)

机构地区：[1]青岛市产品质量检验研究院,山东青岛266000 [2]青岛市食品检验所,山东青岛266000

出　　处：《现代食品》2024年第19期192-195,共4页Modern Food

摘　　要：N-亚硝胺具有强致癌性,保健食品中N-亚硝胺含量的测定鲜有报道。本文利用QuEChERS技术对保健食品中的N-亚硝胺进行提取净化,使用气相色谱-三重四极杆质谱仪分析测试N-亚硝胺类化合物,建立QuEChERS-气相色谱-三重四极杆质谱法同时测定保健食品中8种N-亚硝胺的方法。结果表明,8种N-亚硝胺化合物浓度在0.5~10.0μg·L^(-1)线性良好,线性相关系数均大于0.999,检出限为0.12~0.32μg·kg^(-1),定量限为0.40~1.08μg·kg^(-1),3个浓度水平的平均加标回收率为88.7%~115.4%,相对标准偏差为2.0%~9.5%。利用该方法对市面上常见的10种保健食品进行测定,均未检出8种N-亚硝胺。该方法操作简单、取样量少、灵敏度与精密度良好,适用于测定保健食品中的N-亚硝胺化合物。N-nitrosamines have strong carcinogenicity,and there are few reports on the determination of N-nitrosamine content in health food.This paper uses QuEChERS technology to extract and purify N-nitrosamines in health food,and uses gas chromatography-triple quadrupole mass spectrometer to analyze and test N-nitrosamine compounds.The QuEChERSgas chromatography-triple quadrupole mass spectrometry method is established for the simultaneous determination of eight N-nitrosamines in health food.The experimental results show that the eight N-nitrosamine compounds have good linearity in the concentration range of 0.5 μg·L~(-1) to 10.0 μg·L~(-1),with linear correlation coefficients greater than 0.999.The detection limit is from 0.12 μg·g~(-1) to 0.32 μg·kg^(-1),and the quantification limit is from 0.40 μg·kg^(-1) to 1.08 μg·kg^(-1).The average spiked recovery rates of the three concentration levels is from 88.7% to 115.4%,and the relative standard deviation is from 2.0% to 9.5%.The method is used to determine ten kinds of common health food in the market,and eight kinds of N-nitrosamines are not detected.This method has the advantages of simple operation,small sampling amount,good sensitivity and precision,and is suitable for the determination of N-nitrosamines compounds in health foods.

关 键 词：N-亚硝胺 保健食品 QUECHERS 气相色谱-三重四极杆质谱法 

分 类 号：TS251.52[轻工技术与工程—农产品加工及贮藏工程]