大豆多糖的提取、纯化及其结构的测定研究  

Extraction and Purification of Soybean Polysaccharides and Determination of Their Structure

作　　者：万振平 张鸿雁 李碧珠 于培玲 黄立新[2] WAN Zhenping;ZHANG Hongyan;LI Bizhu;YU Peiling;HUANG Lixin(Guangzhou Shuangqiao Co.,Ltd.,Guangzhou 510280,China;College of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]广州双桥股份有限公司,广东广州510280 [2]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《现代食品》2024年第19期205-210,222,共7页Modern Food

基　　金：华南理工大学2024年学生研究计划项目(X202410561609);2024年“百步梯攀登计划”项目(j2tw202402106)。

摘　　要：大豆多糖是一种从豆渣中提取的水溶性多糖,具有乳化稳定性、抗氧化、降血脂、降血糖和调节肠道菌群等多种生物活性,被广泛应用于食品、医药、保健等行业。本文以磷酸溶液为溶剂提取豆渣中的大豆多糖,在单因素试验的基础上进行正交优化,并进行纯化与结构测定研究。结果表明,大豆多糖的最佳提取工艺为固液比1∶11,pH值3.5~4.0,提取时间4 h,提取温度140℃。纯化后的大豆多糖呈无定型态,单糖组成有半乳糖、鼠李糖和阿拉伯糖,其平均分子量约为314.36 kDa。Soybean polysaccharide is a water-soluble polysaccharide extracted from soya bean dregs,which has various biological activities such as emulsion stability,antioxidant,lipid-lowering,hypoglycemic,and regulation of gut microbiota.It is widely used in industries such as food,medicine,and healthcare.In this paper,aqueous phosphoric acid solution was used as a solvent to extract water-soluble soybean polysaccharides from soya bean dregs,orthogonal optimization was carried out on the basis of single factor experiment,and the purification and structure determination were conducted.The results showed that the optimum preparation conditions of water-soluble soybean polysaccharide were as follows the solid-liquid ratio was 1∶ 11,the pH value was 3.5~ 4.0,the extration time was 4 h,and the extraction temperature was 140 ℃.The purified soybean polysaccharide exhibited an amorphous structure,and its monosaccharide composition primarily consisted of galactose,rhamnose and arabinose,with an average molecular weight of 314.36 kDa.

关 键 词：大豆多糖 提取 纯化 结构 

分 类 号：R284.2[医药卫生—中药学]