乌兰察布地区马铃薯疮痂病新型病原菌种类及致病性鉴定  

Species and Pathogenicity Identification of Streptomyces Species Causing Potato Common Scab in Ulanqab Area

作　　者：王越 曹春梅 陈汉 王晓娇 余乾鹏 李学洋 张志凯 胡柏耿 WANG Yue;CAO Chunmei;CHEN Han;WANG Xiaojiao;YU Qianpeng;LI Xueyang;ZHANG Zhikai;HU Baigeng(National Engineering Research Center for Potato,Leling,Shandong 253600;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Huhhot,Inner Mongolia 010031;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095;Leling Xisen Potato Industry Co.Ltd.,Leling,Shandong 253600)

机构地区：[1]国家马铃薯工程技术研究中心,山东乐陵253600 [2]内蒙古自治区农牧业科学院,呼和浩特010031 [3]南京农业大学植物保护学院,南京210095 [4]乐陵希森马铃薯产业集团有限公司,山东乐陵253600

出　　处：《中国农学通报》2024年第32期151-156,共6页Chinese Agricultural Science Bulletin

基　　金：山东省重大专项(重大科技创新工程)(2022CXGC01060);山东省重大专项(农业良种工程)(2022LZGC017)。

摘　　要：研究旨在对土壤中马铃薯病原菌分离鉴定,明确致病基因及致病能力。对开展抗疮痂病育种及综合防治具有重要意义。对采集的发病薯块,采用组织分离法和涂布稀释、通过16S r DNA测序进行种类鉴定并构建系统发育树,PCR鉴定致病基因,萝卜幼苗法、小薯片法和盆栽接种鉴定测试致病性。共分离鉴定致病性链霉菌4种,分别为Streptomyces scabies、Streptomyces thermocarboxydus、Streptomyces rubrogriseus、Streptomyces kanamyceticus。以上菌株分别接种希森3号进行致病性检测,测试结果表明,上述4种链霉菌均能引起块茎发病,且致病基因组合均为txtAB+/tomA+/nec1+;本研究共鉴定马铃薯疮痂病致病性链霉菌4种,除Streptomyces scabies外,Streptomyces thermocarboxydus、Streptomyces rubrogriseus、Streptomyces kanamyceticus 3种病原菌为马铃薯疮痂病新型致病菌。This research aimed to isolate and identify potato common scab pathogens in soil,and clarify the pathogenic genes and pathogenicity.It is of great significance to carry out breeding and comprehensive prevention and control of scab disease.The collected diseased tubers were identified by tissue separation and coating dilution,and the phylogenetic tree was constructed by 16S rDNA measurement.The pathogenic genes were identified by PCR,and the pathogenicity was tested by radish seedling method,small potato chip method and pot inoculation.Four species of pathogenic Streptomyces were isolated and identified as Streptomyces scabies,Streptomyces thermocarboxydus,Streptomyces rubrogriseus and Streptomyces kanamyceticus.All strains were inoculated with‘Xisen 3’for pathogenicity test.The test results showed that four Streptomyces could cause tuber disease,and the pathogenic gene combination was txtAB+/tomA+/nec1+;in this study,a total of 4 pathogenic Streptomyces species were identified.Streptomyces thermocarboxydus,Streptomyces rubrogriseus and Streptomyces kanamyceticus were new pathogens of potato common scab.

关 键 词：马铃薯 疮痂病 致病性链霉菌 分离鉴定 致病基因 16S rDNA测序 系统发育树 PCR鉴定 

分 类 号：S432[农业科学—植物病理学]