鸢尾苷对晚期糖基化终末产物形成的抑制作用  

Inhibitory Effect of Tectoridin on the Formation of Advanced Glycation End Products

作　　者：郭芳瑜 张南海 邹晴如 李广辉 平青玲 陈媛 姜涛 GUO Fangyu;ZHANG Nanhai;ZOU Qingru;LI Guanghui;PING Qingling;CHEN Yuan;JIANG Tao(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004

出　　处：《食品工业科技》2024年第24期98-106,共9页Science and Technology of Food Industry

基　　金：江西省教育厅科技项目基金(GJJ211238);江西省教育厅科技项目基金(GJJ2200957);国家自然科学基金(22368026);2023年江西省大学生创新创业训练计划(S202310412106)。

摘　　要：从食源性天然产物中寻找活性高、毒副作用小的晚期糖基化终末产物(AGEs)抑制剂,对预防和改善相关疾病具有重要意义。为阐明鸢尾苷对晚期糖基化终末产物(AGEs)生成的抑制作用,本研究建立了牛血清白蛋白(BSA)-甲基乙二醛(MGO)糖基化反应模型,采用光谱法、十二烷基硫酸钠-丙烯酰胺凝胶电泳(SDSPAGE)、高效液相色谱(HPLC)、液质联用(LTQ-Orbitrap MS)、分子对接等实验测定了鸢尾苷对AGEs抑制率和蛋白质糖基化程度、氧化产物、交联情况及MGO捕获能力的影响。结果表明,鸢尾苷能提高体系中游离赖氨酸和蛋白质巯基含量,降低蛋白质羰基和氧化产物含量,减少BSA交联结构。鸢尾苷和MGO孵育体系中鉴定出鸢尾苷-MGO单加合物。分子对接结果显示,鸢尾苷可能在氢键和疏水相互作用的驱动下与BSA结合形成复合物。因此,鸢尾苷可作为良好的AGEs抑制剂,其机制可能是鸢尾苷捕获MGO形成鸢尾苷-MGO单加合物,同时与BSA结合形成鸢尾苷-BSA复合物,进而抑制AGEs的生成。本研究结果可为鸢尾苷作为新型、天然、有效的AGEs抑制剂的开发提供一定的理论依据。It is of great significance to search for advanced glycation end products(AGEs)inhibitors with high activity and low side effects from food-borne natural products for the prevention and improvement of related diseases.To elucidate the inhibitory effect of tectoridin on the generation of advanced glycation end products(AGEs),the glycation system of bovine serum protein(BSA)-methylglyoxal(MGO)was established,the effects of tectoridin on the AGEs inhibition,glycation degree,protein oxidation products,protein cross-linking and MGO trapping were investigated by spectroscopy,SDS-PAGE,HPLC,LTQ-Orbitrap MS,and molecular docking.The results showed that tectoridin increased the content of lysine and thiol,reduced the contents of protein carbonylation and protein oxidation products,alleviated the cross-linking structure of BSA.The mono-MGO adduct of tectoridin was identified in the incubation of MGO with tectoridin.Molecular docking results showed that hydrogen bonding and hydrophobic interactions were the major driving forces for stabilizing tectoridin-BSA complexes.Tectoridin was a promising natural AGEs inhibitor.Mechanism analysis revealed that tectoridin alleviated AGEs formation by trapping MGO to form mono-tectoridin-MGO complexes and forming stable tectoridin-BSA complexes.These findings would provide the theoretical basis for the development of tectoridin as a novel,natural and effective inhibitor of AGEs.

关 键 词：鸢尾苷 晚期糖基化终末产物 牛血清白蛋白 甲基乙二醛 抑制机制 

分 类 号：TS201.2[轻工技术与工程—食品科学]