核桃粕多酚提取物预防高尿酸血症的作用及机制  

Prevention and Mechanisms of Walnut Meal Polyphenol Extract on Hyperuricemia

作　　者：徐冬月 马雅鸽 牛若楠 赵声兰 XU Dongyue;MA Yage;NIU Ruonan;ZHAO Shenglan(College of Chinese Medicine,Yunnan University of Traditional Chinese Medicine,Kunming 650201,China;College of Traditional Chinese Medicine and Health,Beihai Vocational of Wellness,Beihai 536000,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650201 [2]北海康养职业学院中医健康学院,广西北海536000

出　　处：《食品工业科技》2024年第24期328-338,共11页Science and Technology of Food Industry

基　　金：云南省永平县核桃产业科技特派团(202204BI090014);云南省重大科技专项(202102AE090031,202402AE090011)。

摘　　要：采用高尿酸血症(hyperuricemia,HUA)小鼠模型和边造模边灌胃造模药和核桃粕多酚提取物的方法,考察核桃粕多酚提取物预防HUA作用及机制。以30 g/kg酵母膏灌胃(ig)和300 mg/kg氧嗪酸钾腹腔注射(ip)联合给药建立高尿酸血症小鼠模型,检测血清尿酸(UA)、肌酐(Cr)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平和黄嘌呤氧化酶(XOD)、谷草转氨酶(AST)及谷丙转氨酶(ALT)活性,苏木素-伊红染色观测分析肾脏病理状况,LC-MS技术检测肝脏代谢组,Illumina技术检测小鼠肠道菌群。结果显示,与模型组相比,核桃粕多酚提取物各剂量预防组能显著降低肾脏指数、血清Cr、IL-6、UA水平及XOD、ALT、AST活力,除低剂量组UA的P<0.05外,其它各组P<0.0001;高、中剂量预防组能显著降低TNF-α(P<0.01),预防组肾脏病变及肾小管空泡化减轻、肠道优势菌乳杆菌丰度增加。鉴定到20个差异代谢物,核桃粕多酚提取物预防HUA的肝脏主要生物标志物为犬尿酸和溶血磷脂,差异代谢主要涉及调控TCA循环、铁载体群非核糖体肽生物合成、牛磺酸和次牛磺酸代谢等通路。综上,核桃粕多酚提取物能显著预防高尿酸血症,其机制可能是有效降低血清XOD活性,阻止建模诱发的小鼠肠道优势乳杆菌相对丰度下降和调控肝脏代谢,有效降低肝脏溶血磷脂(P<0.05)和增加犬尿酸(P<0.05)。To study the effect and mechanism of walnut meal polyphenol extract on hyperuricemia(HUA),method of filling the corresponding mold making drug and walnut meal polyphenolic extract,a mouse model of hyperuricemia(HUA)was established by co-administration of 30 g/kg yeast paste by gavage(ig)and 300 mg/kg potassium oxonate by intraperitoneal injection(ip),and by the method of side-administration of the candidate and modeling drug.The serum uric acid(UA)and creatinine(Cr)of mice were detected,tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)levels and xanthine oxidase(XOD),aspartate aminotransferase(AST)and alanine transaminase(ALT)activities in mice,hematoxylin-eosin staining of kidneys for observation and evaluation of pathology,LC-MS technique for liver metabolome,and Illumina technique for intestinal flora in mice.Results showed that,compared to the model group,renal index,serum UA,Cr,IL-6 levels and the activity of XOD,AST and ALT were significantly prevented from increasing in each subject group(P<0.0001 except P<0.05 of UA in low dose group).TNF-αlevels were significantly prevented from increasing in the high and medium dose groups(P<0.01),and renal lesions and renal tubular vacuolization were reduced,and the abundance of the dominant intestinal Lactobacillus were increased.There were 20 differential metabolites including biomaker kynurenic acid and lysoPA,which mainly involved in regulating pathways such as TCA cycle,biosynthesis of siderophore group nonribosomal peptides,taurine and hypotaurine metabolism.In summary,the polyphenol extract from walnut meal can significantly prevent hyperuricemia.The mechanism may involve effectively reducing serum XOD activity,preventing the decrease in relative abundance of dominant Lactobacillus in the intestine of mice induced by modeling,regulating liver metabolism,effectively lowering lysoPA(P<0.05),and increasing kynurenic acid(P<0.05).

关 键 词：高尿酸血症 核桃粕多酚提取物 预防 代谢组 肠道菌群 

分 类 号：R151.2[医药卫生—营养与食品卫生学]