导致一起食物中毒的产气荚膜梭菌的病原学特征和全基因组序列分析  

Etiological characteristics and whole genome sequence analysis of Clostridium perfringens causing a food poisoning outbreak

作　　者：弋英[1] 郭凯[1] 肖华 周彤 张毅 刘晓琳[1] Yi Ying;Guo Kai;Xiao Hua;Zhou Tong;Zhang Yi;Liu Xiaolin(Qingdao Center for Disease Control and Prevention of Bacterial Disease Laboratory/Qingdao Institute of Prevention Medicine,ShandongQingdao266033,China;School of Public Health Zunyi Medical University,Key Laboratory of Maternal&Child Health and Exposure Science of Guizhou Higher Education Institutes,Zunyi563000,China)

机构地区：[1]青岛市疾病预防控制中心细菌性疾病检验所,青岛市预防医学研究院,青岛266032 [2]遵义医科大学公共卫生学院,贵州省高等学校孕妇儿童健康与暴露科学重点实验室,遵义563000

出　　处：《中华预防医学杂志》2024年第11期1789-1795,共7页Chinese Journal of Preventive Medicine

基　　金：贵州省科技计划项目(黔科合基础-ZK(2023)重点057;黔科合平台人才-CXTD(2022)014);贵州省高等学校重点实验室研究项目(黔教技(2023)019号)。

摘　　要：采用多重实时荧光PCR对一起食物中毒所采集的样本进行病原菌初筛;根据初筛结果进行可疑病原菌产气荚膜梭菌(CP)的分离培养与鉴定;对CP分离株、肛拭标本及食品样品进行CP相关毒力基因的核酸检测;采用脉冲场凝胶电泳对CP分离株进行分子分型分析;通过对plc基因测序,构建系统进化树,分析核苷酸和氨基酸同源性;对其代表性菌株QD2022FB4-CP4进行全基因组测序和基因注释,探索其耐药性、毒素基因以及生物学特征。通过上述分析发现该起食物中毒由F型产气荚膜梭菌导致,该菌通过污染肉夹馍进而引起食用人群感染;通过分析QD2022FB4-CP菌株全基因组序列,发现其代谢活动较活跃、含有多种毒力基因,并存在多重耐药的特点。This study investigated the etiological characteristics and whole genome sequencing of clostridium perfringens(CP)from a food poisoning outbreak.Multiplex real-time PCR was employed to screen pathogens in collected samples.Based on the preliminary screening results,the isolation,cultivation,and identification of suspected pathogenic CP bacteria were performed.The nucleic acid tests were conducted for CP-related virulence genes on CP isolates,anal swab specimens and food samples.The molecular typing of CP isolates was analyzed by using pulsed-field gel electrophoresis(PFGE).A phylogenetic tree was established to analyze nucleotide and amino acid homology after sequencing the PLC gene.The whole genome sequencing and gene annotation on the representative strain QD2022FB4-CP4 were performed to explore its drug resistance,toxin genes and biological characteristics.The analysis revealed that the food poisoning was triggered by F-type Clostridium perfringens,which infected the consumers by contaminating‘roujiamo’sandwiches.The whole genome sequencing of the strain QD2022FB4-CP found that it had active metabolic processes,multiple virulence genes and multidrug resistance characteristics.

关 键 词：产气荚膜梭菌 食物中毒 脉冲场凝胶电泳 全基因组序列分析 

分 类 号：R595.7[医药卫生—内科学] R446.5[医药卫生—临床医学]