Organoids in concert:engineering in vitro models toward enhanced fidelity  

作　　者：Zhengkun Chen Ryohichi Sugimura Yu Shrike Zhang Changshun Ruan Chunyi Wen 

机构地区：[1]Department of Chemistry,University of Toronto,Toronto,Ontario,Canada [2]Centre for Translational Stem Cell Biology,Hong Kong SAR,China [3]Li Ka Shing Faculty of Medicine,University of Hong Kong,Hong Kong SAR,China [4]Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Cambridge,Massachusetts,USA [5]Research Center for Human Tissue and Organs Degeneration,Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,China [6]Department of Biomedical Engineering,Faculty of Engineering,The Hong Kong Polytechnic University,Hong Kong SAR,China [7]Research Institute of Smart Ageing,The Hong Kong Polytechnic University,Hong Kong SAR,China

出　　处：《Aggregate》2024年第2期99-111,共13页聚集体（英文）

基　　金：Health and Medical Research Fund Scheme,Grant/Award Numbers:01150087,16172691;Research Grants Council of Hong Kong ECS,Grant/Award Number:PolyU 251008/18 M;GRF,Grant/Award Numbers:PolyU 151061/20 M,PolyU15100821M;NFSC/RGC schemes,Grant/Award Number:N_PolyU 520/20;ITF MHKJFS,Grant/Award Numbers:MHP/011/20,MHP/037/23,2023YFE0210500;Hong Kong Polytechnic University Project of Strategic Importance,Grant/Award Number:ZE2C;Brigham Research Institute。

摘　　要：Organoids have emerged as a powerful platform for studying complex biological processes and diseases in vitro.However,most studies have focused on individual organoids,overlooking the inter-organ interactions in vivo and limiting the physiological relevance of the models.To address this limitation,the development of a multi-organoid system has gained considerable attention.This system aims to recapitulate inter-organ communication and enable the study of complex physiological processes.This review provides a comprehensive overview of the recent advancements in organoid engineering and the emerging strategies for constructing a multi-organoid system.First,we highlight the critical mechanical,structural,and biochemical factors involved in designing suitable materials for the growth of different organoids.Additionally,we discuss the incorporation of dynamic culture environments to enhance organoid culture and enable inter-organoid communication.Furthermore,we explore techniques for manipulating organoid morphogenesis and spatial positioning of organoids to establish effective inter-organoid communication networks.We summarize the achievements in utilizing organoids to recapitulate inter-organ communication in vitro,including assembloids and microfluidic multiorganoid platforms.Lastly,we discuss the existing challenges and opportunities in developing a multi-organoid system from its technical bottlenecks in scalability to its applications toward complex human diseases.

关 键 词：assembloids BIOMATERIALS in vitro models MICROFLUIDICS ORGANOIDS 

分 类 号：O62[理学—有机化学]