基于中红外光谱的牛乳中A 2β-酪蛋白检测方法研究  

Detection of A2β-casein in Milk Based on Mid-infrared Spectroscopy

作　　者：杨戬 刘伯扬[1] 王丹慧[1] 高永亮[1] 赵三军 赵凯 李慧[1] 仪虹伯 YANG Jian;LIU Boyang;WANG DanHui;GAO Yongliang;ZHAO Sanjun;ZHAO Kai;LI Hui;YI Hongbo(Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Hohhot Inner Mongolia 011517)

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011517

出　　处：《中国乳业》2024年第11期144-148,156,共6页China Dairy

基　　金：呼和浩特市科技计划项目(2021-社-5);呼和浩特市科技计划项目(2016-高新-4,2021-农-重-1)。

摘　　要：[目的]研究基于中红外光谱的牛乳中A2β-酪蛋白检测方法。[方法]进行光谱采集,利用多元散射校正及标准正态化预处理提高光谱信噪比,在竞争自适应重加权算法筛选得到特征变量基础上,建立基于偏最小二乘的定量分析模型。[结果]预处理及变量筛选后,训练、预测误差分别达到0.061 g/100 g、0.068 g/100 g,R分别达到0.893、0.875。[结论]中红外光谱方法可用于牛乳中A2β-酪蛋白快速检测,光谱预处理、特征变量提取方法显著提高建模精度,可为牛乳中其他成分的光谱检测方法准确性提供参考。[Objective]A method for the detection of A2β-casein in milk based on mid-infrared spectroscopy is investigated.[Method]The MIR spectrum of milk samples were measured and preprocessed with multiple scattering correction and standard normalization to improve the signal-to-noise ratio.Then,the characteristic variables were selected by the competitive adaptive reweighting algorithm.The quantitative analysis model based on partial least squares was established.[Result]After pre-processing and variable screening,the training and prediction errors reached 0.061 g/100 g and 0.068 g/100 g,respectively,and the R reached 0.893 and 0.875,respectively.[Conclusion]The mid-infrared spectroscopy method can be used for the rapid detection of A 2β-casein in milk.The spectral pre-processing and characteristic variables extraction methods can significantly improve the modelling accuracy.It also provided a good basis for the spectral detection methods for other ingredients in milk.

关 键 词：中红外光谱 A2β-酪蛋白 预处理 竞争自适应重加权采样 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.33[轻工技术与工程—食品科学与工程]