高效液相色谱分析中药肉桂多酚提取物中的3种多酚成分  

Determination three polyphenol components in polyphenol extract of Cinnamomum cassia Presl by high performance liquid chromatography

作　　者：郑玉 史丹阳 龚灿 许旭 高建平[2] ZHENG Yu;SHI Danyang;GONG Can;XU Xu;GAO Jianping(School of Chemical and Environmental Engineering,Shanghai Institute of Technology,Shanghai 201418,China;School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418 [2]上海中医药大学中药学院,上海201203

出　　处：《应用技术学报》2024年第4期390-394,共5页Journal of Technology

基　　金：国家自然科学基金面上项目(82074055)资助。

摘　　要：建立了中药肉桂多酚提取物中没食子酸、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯3种成分的高效液相色谱分析方法。采用Kromasil 100-3.5 C_(18)色谱柱(4.6 mm×150 mm),流动相为0.2%三氟乙酸水溶液(A)和甲醇-水溶液(80∶20)(B)梯度洗脱(30%~80%B),流速为1.0 mL/min,检测波长为278 nm。日内精密度小于0.3%。没食子酸(0.359~368μg/mL)、表儿茶素没食子酸酯(0.629~629μg/mL)、表没食子儿茶素没食子酸酯(1.02~1041μg/mL)在各自浓度范围内的线性相关系数均达到0.9999,3种成分的检测限分别为0.07、0.17和0.26μg/mL。将方法用于测定2种总酚含量相近的肉桂多酚实际样品,显示2种样品的成分存在明显差异。该分析方法操作简便、精确可靠,适用于肉桂多酚提取物成分组成的分析及其中3种多酚成分的定量测定。A high performance liquid chromatography(HPLC)method was developed for the determination of three components,namely,gallic acid,epigallocatechin gallate(EGCG),and epicatechin gallate(ECG),in the polyphenolic extract of Cinnamomum cassia.The HPLC method was performed using a Kromasil 100-3.5 C_(18)column(4.6 mm×150 mm)with mobile phases consisting of 0.2%trifluoroacetic acid aqueous solution(A)and methanol-aqueous solution(80:20)(B)with gradient elution(30%-80%B).The flow rate was set at 1.0 mL/min,and the detection wavelength was 278 nm.The intraday precision of the method was found to be less than 0.3%.The linear correlation coefficients of gallic acid(0.359-368μg/mL),EGCG(0.629-629μg/mL),and ECG(1.02-1041μg/mL)in their respective concentration ranges could reach 0.9999,and the detection limits of the three components were 0.07,0.17 and 0.26μg/mL,respectively.The method was applied to determine two actual samples of cinnamon polyphenol extract with similar total phenol content,revealing significant differences in their compositions.This analytical method is simple to operate,precise,and reliable,making it suitable for the analysis of the composition of cinnamon polyphenolic extracts and the quantitative determination of the three polyphenolic components in cinnamon polyphenol extract.

关 键 词：肉桂 多酚 高效液相色谱法 表没食子儿茶素没食子酸酯 

分 类 号：O657.72[理学—分析化学] R917[理学—化学]