聚二乙炔囊泡用于饮料酒中乙醇的可视化检测  

Visual Detection of Ethanol in Alcoholic Beverages Based on Polydiacetylene Vesicles

作　　者：陈荦 张雨婷 王晓丽[1,2] 周楠迪 CHEN Luo;ZHANG Yuting;WANG Xiaoli;ZHOU Nandi(School of Biotechnology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学糖化学与生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2024年第8期50-57,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(42177212,21877054)。

摘　　要：随着人们生活水平的提升,饮料酒的消费逐渐上升,引起了市场监管和更多消费者对其品质和乙醇检测方面的关注。因此开发一种操作简单、检测迅速且结果直观的新型乙醇检测器十分必要。作者设计并制备了用于检测饮料酒中乙醇的聚二乙炔(polydiacetylene,PDA)囊泡,通过饮料酒中乙醇对PDA囊泡结构的扰动和基团的作用,使囊泡由无乙醇存在时的蓝色逐渐转变为有乙醇时的红色,从而检测产品的乙醇体积分数。结果表明,乙醇体积分数为30%~90%时,与PDA囊泡的比色响应(colorimetric response,CR)值具有良好的线性关系,在实际酒样检测中的相对标准偏差为1.45%~2.23%,裸眼识别颜色变化效果较好。此方法具有高效便捷、结果可视化、反应迅速的优点,为饮料酒中乙醇的检测提供了新方法。As the quality of life improves,the consumption of alcoholic beverages is gradually increasing,leading to heightened market supervision and consumer focus on quality and ethanol testing.Consequently,there is a pressing need to develop novel ethanol detector that is simple to operate,provides rapid detection,and yields intuitive results.The author has designed and developed polydiacetylene(PDA)vesicles for detecting ethanol volume fraction in beverages.The ethanol in the alcoholic beverage disrupts the structure of the PDA vesicles and interacts with their functional groups,causing a gradual color change in the vesicles from blue in the absence of ethanol to red in its presence,thereby allowing for the detection of the ethanol volume fraction in the product.The results demonstrated a good linear relationship between the ethanol volume fraction(30%~90%)and the colorimetric response(CR)of the PDA vesicles.The relative standard deviation in real wine sample detection ranged from 1.45%~2.23%,with color changes being easily detectable to the naked eye.This method is both efficient and convenient,offering the advantages of visual accessibility and rapid response,thereby providing a novel approach for ethanol detection in alcoholic beverages.

关 键 词：饮料酒 聚二乙炔囊泡 可视化检测 

分 类 号：TS262[轻工技术与工程—发酵工程] O657.3[轻工技术与工程—食品科学与工程]