液相色谱-串联三重四极杆质谱仪快速测定鱼腥草中152种农药残留  

Rapid determination of 152 pesticide residues in Houttuynia cordata by liquid chromatography tandem triple quadrupole mass spectrometer

作　　者：彭汝林 刘媛媛 李海波 杜业刚[1] 罗淙岚 朱雨田[1] PENG Ru-lin;LIU Yuan-yuan;LI Hai-bo;DU Ye-gang;LUO Cong-lan;ZHU Yu-tian(Shenzhen Academy of Metrology and Quality Inspection,Shenzhen 518131,Guangdong,China;College of Food Science,South China Agricultural University,Guangzhou 510642,Guangdong,China)

机构地区：[1]深圳市计量质量检测研究院,广东深圳518131 [2]华南农业大学食品学院,广东广州510642

出　　处：《粮食与油脂》2024年第12期154-162,共9页Cereals & Oils

基　　金：国家重点研发计划(2022YFF0607900);深圳市自然科学基金(JCYJ20210324141809025);深圳市可持续发展专项(KCXFZ20230731092859013)。

摘　　要：建立QuEChERS结合液相色谱-串联三重四极杆质谱仪快速测定鱼腥草中152种农药残留的方法。样品用10 mL乙腈提取,经过分散固相萃取净化,以含体积分数0.01%甲酸的5 mmol/L乙酸铵溶液(A)与甲醇(B)作为流动相进行梯度洗脱,采用多反应监测模式(MRM)进行定性定量分析。结果表明:152种农药在质量浓度0.001~0.100 mg/kg范围内具有良好的线性关系,相关系数均大于0.995,检出限为3.2×10^(-3)~0.843μg/kg,定量限为1.97×10^(-3)~2.81μg/kg,回收率为63.7%~109.2%,相对标准偏差(RSD,n=6)为0.42%~12.60%。A method for rapid determination of 152 pesticide residues in Houtuynia cordata using QuEChERS combined with liquid chromatography tandem triple quadrupole mass spectrometry was estab-lished.The sample was extracted with 10 mL of acetonitrile and purified by dispersed solid-phase extrac-tion.A gradient elution was performed using 5 mmol/L ammonium acetate solution containing volume frac-tion 0.01%formic acid(A)and methanol(B)as the mobile phase,and qualitative and quantitative analy-sis was performed using multiple reaction monitoring mode(MRM).The results showed that 152 pesticides had a good linear relationship within the mass concentration range of 0.001-0.100 mg/kg,with correla-tion coefficients greater than 0.995.The limit of detection was 3.2×10^(-3)-0.843μg/kg,and the limit of quantitotion was 1.97×10^(-3)-2.81μg/kg.The recovery rate was 63.7%-109.2%,and the relative standard deviation(RSD,n=6)was 0.42%-12.60%.

关 键 词：QUECHERS 分散固相萃取 液相色谱-串联三重四极杆质谱 鱼腥草 农药残留 

分 类 号：TS207.53[轻工技术与工程—食品科学]