刺松藻可溶性膳食纤维的提取及理化性质的研究  

Extraction and Physicochemical Characterization of Soluble Dietary Fiber from Holothuria scabra

作　　者：李滨涵 王婧[1] 李嘉俊 贾文睿 牛小月 张欢 梁博 LI Binhan;WANG Jing;LI Jiajun;JIA Wenrui;NIU Xiaoyue;ZHANG Huan;LIANG Bo(Harbin University of Science and Technology,Harbin,Heilongjiang 150080,China;Jiangsu College of Engineering and Technology,Nantong,Jiangsu 226006,China)

机构地区：[1]哈尔滨理工大学,黑龙江哈尔滨150080 [2]江苏工程职业技术学院,江苏南通226006

出　　处：《农产品加工》2024年第21期54-58,共5页Farm Products Processing

基　　金：黑龙江大学生创新创业项目(S202310214112)。

摘　　要：以刺松藻为原料,采用生物酶解法提取刺松藻可溶性膳食纤维。通过单因素试验和正交试验,确定纤维素酶酶解法提取刺松藻SDF的最佳工艺条件,测定所得刺松藻可溶性膳食纤维的理化性质。结果表明,酶解pH值6.0,料液比1∶25,酶解温度45℃,酶解时间3 h,酶添加量15%,提取率为88.2%,此时刺松藻SDF颜色为类白色,持水力、持油力、溶解性分别为(4.7430±0.1487)g/g,(1.4800±0.0092)g/g,(84.40±0.56)%;以上研究表明,刺松藻可溶性膳食纤维具有较好的理化功能特性,该研究为刺松藻的高值化利用提供了理论依据。In this study,soluble dietary fiber(SDF)was extracted from Holothuria scabra using an enzymatic hydrolysis method with cellulase.The optimal extraction conditions were determined through single-factor and orthogonal experiments.The physicochemical properties of the extracted SDF were also investigated.The results showed that the highest extraction yield(88.2%)were achieved under the following conditions:initial pH 6.0,liquid-to-solid ratio 1∶25,temperature 45℃,enzymatic hydrolysis time 3 h,and enzyme addition at 15%,the extraction volume reached 88.2%.The color of the extracted SDF was off-white.Furthermore,the water holding capacity,oil holding capacity,and solubility of the SDF from Holothuria scabra were found to be(4.7430±0.1487)g/g,(1.4800±0.0092)g/g,and(84.40±0.56)%,respectively.These findings demonstrated that soluble dietary fiber derived from Holothuria scabra exhibits favorable physicochemical functional characteristics which could contribute to its high-value utilization in various applications.

关 键 词：刺松藻 可溶性膳食纤维(SDF) 纤维素酶 理化特性 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]