响应面超声辅助法优化亳白芷粗多糖提取工艺及抗氧化研究  

Optimization of Crude Polysaccharide Extraction Process and Antioxidant Activity of Bobai Zhi Using Response Surface Ultrasound Assisted Method

作　　者：朱勇 陈群[1,2] 丁锐 张元波 程竹青[2,3] ZHU Yong;CHEN Qun;DING Rui;ZHANG Yuanbo;CHENG Zhuqing(Bozhou Branch,Anhui Academy of Traditional Chinese Medicine,Bozhou Anhui 236800,China;School of Pharmacy,Bozhou Vocational and Technical College,Bozhou Anhui 236800,China;School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230000,China)

机构地区：[1]安徽省中医药科学院亳州分院,安徽亳州236800 [2]亳州职业技术学院药学院,安徽亳州236800 [3]安徽中医药大学药学院,安徽合肥230000

出　　处：《文山学院学报》2024年第5期8-13,25,共7页Journal of Wenshan University

基　　金：安徽省高等学校自然科学研究重点项目“基于响应面法对亳白芷多糖提取工艺及抗氧化活性研究”(2023AH053211);安徽省高等学校质量工程教学研究项目“浅谈新形势线下高职中药类专业药用基础化学实验教学的改革”(2022jyxm1035)。

摘　　要：为了获得亳白芷粗多糖最佳提取工艺和抗氧化性参数,采用超声波辅助下设计三因素三水平响应面试验,优化亳白芷多糖提取工艺,并对DPPH、羟基自由基的清除能力进行测定,研究亳州白芷粗多糖抗氧化活性。结果显示:在液料比59:1(mL/g)、超声温度为72℃、超声时间为81 min和超声功率为250 W条件下多糖提取率最高为8.65%;白芷多糖浓度在一定的范围里与自由基清除率呈量效关系,当多糖浓度为4 mg/mL,羟基自由基清除率为94.39%,DPPH自由基的清除率也可达到85.07%,接近于维生素C阳性对照组。研究表明响应面超声辅助法优化亳白芷粗多糖提取工艺方案设计可行,亳白芷多糖具有较好的抗氧化活性,可作为天然抗氧化剂。In order to obtain the optimal extraction process and antioxidant parameters of crude polysaccharides from Bobai Zhi.Using ultrasound assisted design,a three factor and three level response surface experiment was conducted to optimize the extraction process of polysaccharides from Bobai Zhi;And the scavenging ability of DPPH and·OH free radicals was measured to study the antioxidant activity of Bozhou Baizhi crude polysaccharide.The results showed that the highest polysaccharide extraction rate was 8.65%at a liquid to material ratio of 59:1(mL/g),ultrasound temperature of 72℃,ultrasound time of 81 min,and ultrasound power of 250W;The concentration of Bai Zhi polysaccharide shows a dose-response relationship with free radical scavenging rate within a certain range.When the polysaccharide concentration is 4mg/mL,the hydroxyl radical scavenging rate is 94.39%,and the DPPH free radical scavenging rate can also reach 85.07%,which is close to the VC positive control group.Research has shown that the response surface ultrasound assisted optimization of the extraction process for crude polysaccharides from Angelica dahurica is feasible.Angelica dahurica polysaccharides have good antioxidant activity and can be used as natural antioxidants.

关 键 词：亳白芷 粗多糖 响应面 抗氧化 

分 类 号：TS202.1[轻工技术与工程—食品科学]