外源褪黑素对青皮核桃品质及活性氧代谢的影响  

Effects of Exogenous Melatonin on Quality and Active Oxygen Metabolism of Green Walnut

作　　者：杨蕊 张皓波 龚加顺 付晓萍 林奇 包媛媛 YANG Rui;ZHANG Haobo;GONG Jiashun;FU Xiaoping;LIN Qi;BAO Yuanyuan(Institute of Food Science and Technology,Yunnan Agricultural University,Kunming 650100,China;Yunnan Academy of Agricultural Sciences,Kunming 650000,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650100 [2]云南省农业科学院,云南昆明650000

出　　处：《食品科技》2024年第10期23-30,40,共9页Food Science and Technology

基　　金：云南省永平县核桃产业科技特派团项目(202204BI090014)。

摘　　要：以云南‘大麻’青皮核桃为研究对象,探究褪黑素(Melatonin,MT)结合自发气调包装(Modified atmosphere packaging,MAP)处理对青皮核桃贮藏品质和活性氧代谢的影响。对照组采用蒸馏水处理,结合MAP置于4℃低温贮藏。结果表明:MT有效抑制果实裂果率、软褐率和活性氧自由基的累积,增强果实中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)等抗氧化酶的活性及抗氧化物质的含量,促进核仁可溶性糖和可溶性蛋白的积累,延缓核仁酸价上升,保性持良好贮藏品质。在贮藏期90 d时,0.5 mmol/L MT处理的果实裂果率、丙二醛(Malondialdehyde,MDA)含量、相对电导率分别为42%、0.35μmol/g、56.0%,均显著低于0.25mmol/L MT处理的果实(P<0.05);抗坏血酸(Ascorbic acid,AsA)和还原型谷胱甘肽(Glutathione,GSH)含量分别为501.25 mg/100 g和3.30μmol/g,均显著高于0.25 mmol/L MT处理(P<0.05)。综上所述:MT处理可通过调节采后青皮核桃的活性氧代谢来延缓其衰老进程,且0.5 mmol/L MT的效果更佳。该研究结果可为MT应用和青皮核桃采后保鲜提供理论支持。The effects of exogenous melatonin(MT)combined with spontaneous modified atmosphere packaging(MAP)on quality and active oxygen metabolism of green walnut,Yunnan‘Da Ma'green walnut as the research object.The control group was treated with distilled water and stored at 4℃in combination with MAP.The results showed that the exogenous MT could effectively inhibit the fruit cracking rate,soft brown rate and the accumulation of reactive oxygen species increase,the activities of superoxide dismutase(SOD),catalase(CAT)antioxidant enzymes and the contents of antioxidant substances,while the accumulation of soluble sugar and soluble protein in nucleolus was promoted,and the increase of acid value in nucleolus was delayed,maintain good quality.After 90 days storage,the dehiscence rate,malondialdehyde(MDA)content and relative conductivity were 42%,0.35μmol/L and 56.0%,were significantly lower than those treated with 0.25 mmol/L MT combined with MAP treatment(P<0.05).Contents of ascorbic acid(AsA),glutathione(GSH)were 501.25 mg/100 g,3.30μmol/g,significantly higher than 0.25mmol/L MT treatment(P<0.05).In conclusion,MT could delay the senescence process by regulating the metabolism of reactive oxygen species,and 0.5 mmol/L MT treatment was more effective.It provided theoretical support for application of MT and postharvest preservation of green walnut.

关 键 词：青皮核桃 褪黑素 自发气调 活性氧代谢 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]