慈竹总黄酮提取工艺优化及抗氧化活性分析  

Optimization of Extraction Process of Total Flavonoids from Neosinocalamus affinis and Analysis of Antioxidant Activity

作　　者：朱嘉杰 林捷 刘军 李丽 凌生隆 江志聪 田要美 ZHU Jiajie;LIN Jie;LIU Jun;LI Li;LING Shenglong;JIANG Zhicong;TIAN Yaomei(College of Bioengineering,Sichuan University of Science&Engineering,Yibin 644000,China;Sichuan Yibin Sipo Vinegar Industry Co.,Ltd.,Yibin 644000,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644000 [2]四川省宜宾市思坡醋业有限责任公司,四川宜宾644000

出　　处：《食品科技》2024年第10期247-256,共10页Food Science and Technology

基　　金：宜宾市科技计划项目(2022NY031)。

摘　　要：以慈竹叶、慈竹枝、慈竹秆为原料,以超声提取法为基础,分别采用醇提和水提2种方法提取慈竹中的活性成分,并测定粗提液中总黄酮、多酚、多糖的含量及其抗氧化能力,同时对慈竹叶总黄酮的提取工艺进行优化。结果表明,当提取时间为65 min、乙醇体积分数为77%、料液比为1:26 g/mL时,慈竹叶醇提液的总黄酮含量最高,为2.61%。同时慈竹不同部位中活性成分和抗氧化能力有显著差异(P<0.05)。慈竹叶醇提液中总黄酮和多酚含量最高,分别为2.58%和1.75%;慈竹枝醇提液中多糖含量最高,为4.30%。慈竹各部位醇提液的抗氧化能力显著高于水提液,大小关系为慈竹叶>慈竹枝>慈竹秆。Using the Neosinocalamus affinis leaves,branches,and culms as raw materials,based on ultrasonic extraction method.The active ingredients in Neosinocalamus affinis were extracted using alcohol extraction method and water extraction method respectively.The content of total flavonoids,polyphenols,polysaccharides and their antioxidant capacity in the crude extract were determined.At the same time,the extraction process of total flavonoids in Neosinocalamus affinis leaves was optimized.The results showed that when the extraction time was 65 minutes,the ethanol concentration was 77%,and the solid-liquid ratio was 1:26 g/mL,the total flavonoid content in the alcohol extract of Neosinocalamus affinis leaves was the highest at 2.61%.Meanwhile,there were significant differences in active ingredients and antioxidant capacity among different parts of Neosinocalamus affinis(P<0.05);The total flavonoid and polyphenol contents in extract of Neosinocalamus affinis leaves were the highest,at 2.58%and 1.75%,respectively.The highest polysaccharide content was found in the alcohol extract of Neosinocalamus affinis branch,which was 4.30%.The antioxidant capacity of alcohol extracts from various parts of Neosinocalamus affinis was significantly higher than that of water extracts,with a ranking of Neosinocalamus affinis leaves>branches>culms.

关 键 词：慈竹 总黄酮 响应面法 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]