食源性沙门菌耐药特征及全基因组测序分析  

Antimicrobial resistance characteristics and whole genome sequencing analysis of foodborne Salmonella

作　　者：赵莹 董昕 陈嘉 杜强[1] 屠博文[1] 许晓国[1] ZHAO Ying;DONG Xin;CHEN Jia;DU Qiang;TU Bowen;XU Xiaoguo(Changzhou Center for Disease Control and Prevention,Changzhou,Jiangsu 213022,China)

机构地区：[1]常州市疾病预防控制中心,江苏常州213022

出　　处：《中国热带医学》2024年第11期1376-1382,共7页China Tropical Medicine

基　　金：常州市应用基础研究计划项目(No.CJ20220158,No.CJ20220237);常州市卫生健康青苗人才培养工程资助(No.CZQM2023026);常州市卫生健康委科技项目(No.QN202334)。

摘　　要：目的了解江苏省常州市食源性沙门菌的血清型分布情况、耐药特征及其进化关系,为食源性沙门菌的有效防控提供科学依据。方法对2021—2023年常州市食源性疾病监测哨点医院腹泻患者粪便标本中分离的117株沙门菌进行血清型鉴定、药物敏感性试验及全基因组测序,使用综合抗生素耐药性数据库(Comprehensive Antibiotic Resistance Database,CARD)对耐药基因进行注释和分析,使用PubMLST数据库进行多位点序列分型,通过软件GraphPad Pism 9.5绘制耐药基因热图,使用软件Figtree v1.4.4绘制系统发育树。结果117株沙门菌共鉴定出21种血清型,优势血清型为鼠伤寒沙门菌和肠炎沙门菌。耐药结果显示对氨苄西林(77.78%)的耐药率最高,其次为四环素(58.97%)、氨苄西林/舒巴坦(55.55%)、萘啶酸(51.28%),耐药率均超过50.00%,最主要的耐药谱型为CT-AMP-AMS-NAL。基于全基因组测序数据分析得到61种耐药基因,其中包括氨基糖苷类、β-内酰胺类、四环素类、磺胺类、喹诺酮类等耐药基因。多位点序列测定分型(multilocus sequence typing,MLST)共分析得出23种ST型,主要型别为ST34和ST11,系统发育进化树显示大多数相同血清型的沙门菌同源性较高。结论常州市食源性沙门菌的主要血清型为鼠伤寒沙门菌,以ST34为主,菌株携带多种类型的耐药基因,存在多重耐药现象,在今后的防控工作中需引起重视。全基因组测序技术的应用能够更好地监测沙门菌耐药趋势。Objective To investigate the serotype distribution,antibiotic resistance profiles,and evolutionary relationship of foodborne Salmonella isolates in Changzhou,Jiangsu Province,thereby providing the scientific foundation for the prevention and control of Salmonella related foodborne illnesses.Methods Serotyping,antimicrobial susceptibility testing,and wholegenome sequencing(WGS)were performed on 117 strains of Salmonella isolated from stool specimens of diarrhea patients at the foodborne disease surveillance sentinel hospitals in Changzhou from 2021 to 2023.The Comprehensive Antibiotic Resistance Database(CARD)was used for annotation and analysis of antibiotic resistance genes,and the PubMLST database was used for multilocus sequence typing(MLST).Heatmaps of antibiotic resistance genes were generated using GraphPad Prism 9.5,and phylogenetic trees were constructed using Figtree v1.4.4.Results A total of 21 serotypes of Salmonella were identified from 117 strains of Salmonella,with Salmonella typhimurium and Salmonella enteritidis being the predominant types.The resistance results showed the highest resistance rate to ampicillin(77.78%),followed by tetracycline(58.97%),ampicillin/sulbactam(55.55%),and nalidixic acid(51.28%),with resistance rates exceeding 50%.The most common resistance pattern was CT-AMP-AMS-NAL.Whole-genome sequencing data analysis identified 61 resistance genes,encompassing aminoglycoside,β-lactam,tetracycline,sulfonamide,quinolone,and other resistance determinants.MLST analysis identified 23 sequence types(STs),with ST34 and ST11 being the most prevalent.The phylogenetic analysis revealed high homology among Salmonella strains of the same serotype.Conclusions The dominant serotype of foodborne Salmonella in Changzhou is Salmonella typhimurium,primarily ST34,with strains carrying multiple antibiotic resistance genes,indicating a multidrugresistant phenotype that warrants attention in future surveillance efforts.The implementation of WGS technology enhances the monitoring of antibiotic resistance

关 键 词：沙门菌 血清型 耐药基因 全基因组测序 

分 类 号：R155.5[医药卫生—营养与食品卫生学]